园艺学报 ›› 2020, Vol. 47 ›› Issue (2): 370-382.doi: 10.16420/j.issn.0513-353x.2019-0574

• 研究报告 • 上一篇    下一篇

辣椒 β–酮脂酰辅酶 A 合酶基因家族的鉴定与表达分析

易 婷 1,张志硕 2,汤冰倩 1,谢玲玲 2,*,邹学校 1,3,*   

  1. 1 湖南大学研究生院隆平分院,长沙 410125;2 湖南省蔬菜研究所,长沙 410125;3 湖南农业大学园艺园林学院,长沙 410125
  • 出版日期:2020-02-25 发布日期:2020-02-25
  • 基金资助:

Identification and Expression Analysis of the KCS Gene Family in Pepper

YI Ting1,ZHANG Zhishuo2,TANG Bingqian1,XIE Lingling2,*,and ZOU Xuexiao1,3,*   

  1. 1Longping Branch,Graduate School of Hunan University,Changsha 410125,China;2Hunan Research Institute of Vegetables,Changsha 410125,China;3Horticulture & Landscape College,Hunan Agricultural University,Changsha 410125,China
  • Online:2020-02-25 Published:2020-02-25

摘要: 为深入了解 β–酮脂酰辅酶 A 合酶(β-ketoacyl-CoA synthase,KCS)基因家族在辣椒基因组中的特征,利用生物信息学方法鉴定所有 KCS 基因家族成员,对基因染色体定位、系统发育关系、基因结构、蛋白保守基序和组织表达模式等进行分析,并通过 qRT-PCR 方法检测基因在高温、低温和 NaCl胁迫下的响应。从辣椒基因组中共鉴定出 22 个 KCS 家族基因,其不均匀地分布在辣椒的 9 条染色体上;系统发育分析表明辣椒 KCS 基因家族可分为 4 组,每组含有不同数量的基因;该家族成员含有 0 ~ 3 个内含子,保守基序有 6 ~ 14 个;KCS 家族基因具有不同的表达模式;高温、低温和 NaCl 处理可以抑制或激活该家族成员的表达。

关键词: 辣椒, KCS 基因家族, 基因表达

Abstract: In order to understand the characteristics of the β-ketoacyl-CoA synthase(KCS)gene family in the Capsicum annuum genome deeply,all KCS gene family members were identified by using the bioinformatics methods,hereafter,the genes chromosomal location,phylogenetic relationship,gene structure,protein conserved motifs,tissue expression pattern and response to high temperature,low temperature and NaCl stress were analyzed. The results showed that a total of 22 KCS genes were identified and distributed on 9 chromosomes. Phylogenetic analysis indicated that the CaKCS were divided into 4 groups,and each of them included different numbers of genes which contained 0–3 introns and 6–14 conserved motifs. Each gene in the KCS family has different expression patterns,and the CaKCS could be repressed or activated by high temperature,low temperature and NaCl treatment.

Key words: pepper, KCS gene family, gene expression