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园艺学报 ›› 2020, Vol. 47 ›› Issue (1): 53-62.doi: 10.16420/j.issn.0513-353x.2019-0363

• 研究论文 • 上一篇    下一篇

黄瓜CsSUN和CsLNG1调控果实大小的机理分析

徐 婧,潘玉朋,程智慧*   

  1. 西北农林科技大学园艺学院,陕西杨凌 712100
  • 出版日期:2020-01-25 发布日期:2020-01-25
  • 基金资助:
    国家重点研发计划项目(2016YFD0101705-6)

Mechanism Analysis of Fruit Size Regulating Genes CsSUN and CsLNG1 in Cucumber

XU Jing,PAN Yupeng,and CHENG Zhihui*   

  1. College of Horticulture,Northwest A & F University,Yangling,Shaanxi 712100,China
  • Online:2020-01-25 Published:2020-01-25

摘要: 对黄瓜Q30(CsSUN/CsLNG1)及以其为遗传背景的3个近等基因系材料,即Q30(CsSUN/CsLNG1)、QK1.2-S(Cssun/CsLNG1)、QK2.1-S(CsSUN/Cslng1)、QK1.2+2.1-S(Cssun/Cslng1)进行组织形态、内源激素和转录水平的分析结果表明:与QK1.2-S和QK2.1-S相比,Q30果实最为细长,茎粗最小,植株最高。在果实各发育期,Q30的细胞最小,而细胞密度最大。Q30在开花前6 d的BR/ABA及GA3/ABA显著低于3个近等基因系,随着果实发育差异逐渐缩小。各材料ZT/ABA和IAA/ABA在果实发育各时期基本无显著差异。开花前6 d和开花当天Q30的CsSUN表达量均显著高于QK1.2-S和QK1.2+2.1-S,CsLNG1表达量显著高于QK1.2-S,整体来看也高于QK1.2+2.1-S;与细胞膨胀相关的基因Csa1G422480(木葡聚糖内转葡糖基酶基因)、Csa6G014540(扩张蛋白基因)、BR生物合成基因Csa1G524640和GA3调节基因Csa3G872170的定量分析结果却相反,在子房期Q30中的表达量显著低于3个近等基因系。随着果实发育,Q30的CsSUN表达水平与QK1.2-S和QK1.2+2.1-S差异逐渐缩小,CsLNG1与QK2.1-S和QK1.2+2.1-S差异也逐渐缩小,而Csa1G422480、Csa6G014540、Csa1G524640、Csa3G872170表达水平反而逐渐上升,后期甚至显著高于3个近等基因系。综上所述,CsSUN和CsLNG1控制Q30黄瓜细长果实是由于子房期抑制了细胞增大,导致细胞体积小,细胞密度增大;进入果实迅速增长期后该基因对细胞大小的抑制作用减弱,在原有数量基础上细胞逐渐变大,果实持续增长。

关键词: 黄瓜, 果实大小, CsSUN, CsLNG1

Abstract: Three near-isogenic lines (NILs) under the genetic background of Q30(CsSUN/CsLNG1),including QK1.2-S(Cssun / CsLNG1),QK2.1-S(CsSUN / Cslng1),QK1.2+2.1-S(Cssun/Cslng1),were used in this study. Morphological observation showed that fruits of Q30 are much slender than those of QK1.2-S and QK2.1-S. Besides,Q30 also had smaller stem diameter and higher plant height. Histological observation of fruits at different developmental stages indicated that the fruits of Q30 had the smallest cell size and the highest cell density when compared with QK1.2-S,QK2.1-S and QK1.2+2.1-S. Endogenous hormone test of fruits showed that the BR/ABA and GA3/ABA levels of Q30 were significantly lower than QK1.2-S,QK2.1-S and QK1.2+2.1-S at the stage of six-day before anthesis and this difference gradually decreased with the process of fruit development. While, no significant differences were found for ZT/ABA and IAA/ABA levels among these materials. At the stages of anthesis and six-day before anthesis,the relative expression of CsSUN in Q30 was significantly higher than that of QK1.2-S and QK1.2+2.1-S. The expression level of CsLNG1 in Q30 was also higher than that of QK1.2-S and QK1.2+2.1-S. However, the difference gradually reduced with the development and growth of fruit. At the early development stage,the expression levels of cell expansion related genes Csa1G422480(xyloglucan endotransglucosylase gene)and Csa6G014540(expanded protein gene),BR biosynthesis related gene Csa1G524640 and GA3 regulation involved gene Csa3G872170 were significantly lower in Q30 when compared with QK1.2-S,QK2.1-S and QK1.2+2.1-S. However,the expression levels of Csa1G422480,Csa6G014540,Csa1G524640 and Csa3G872170 in Q30 were gradually increased as the growth of fruit,and even significantly higher than QK1.2-S,QK2.1-S and QK1.2+2.1-S at the later stages. In summary,the slender fruits of Q30 are due to the inhibitory effect of CsSUN and CsLNG1 on fruit cell enlargement in the early stage,which resulted smaller cell size and higher cell density. When fruit development reaches to the rapid growth period,the inhibitory effect of these two genes on cell size is weakened,and the fruit cells gradually expand,which promotes the rapid growth of fruit size.

Key words: cucumber, fruit size, CsSUN, CsLNG1

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