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园艺学报 ›› 2015, Vol. 42 ›› Issue (12): 2373-2382.doi: 10.16420/j.issn.0513-353x.2015-0563

• 果树 • 上一篇    下一篇

FaASR基因超表达促进草莓果实着色

贾海锋*,刘众杰,赵鹏程,崔力文,房经贵   

  1. 南京农业大学园艺学院,南京 210095
  • 出版日期:2015-12-25 发布日期:2015-12-25
  • 基金资助:

    国家自然科学基金项目(31401847);江苏省自然科学基金项目(BK20140707);国家博士后基金项目(2014M561663);中
    央高校基本科研业务费专项资金项目(KJQN201541)

Over-expression of Gene FaASR Promotes Strawberry Fruit Coloring

JIA Hai-feng*,LIU Zhong-jie,ZHAO Peng-cheng,CUI Li-wen,and FANG Jing-gui   

  1. College of Horticulture,Nanjing Agricultural University,Nanjing 210095,China
  • Online:2015-12-25 Published:2015-12-25

摘要:

以‘甜查理’草莓为试材,在克隆草莓ASR同源基因的基础上,对草莓中FaASR基因进行生物信息学、时空表达分析,通过调控草莓果实中ASR基因表达水平,分析果实的着色、蔗糖、ABA、色素和果实硬度等生理指标的变化,以及与色素代谢相关基因CHS、UFGT的表达水平,揭示ASR基因调控草莓果实成熟的机制。草莓ASR含有一个典型的与果实成熟和抗逆有关的ABA/WDS区域,ASR基因过量表达可以促进草莓果实提前上色,促进果实内源ABA积累、蔗糖含量增加和果实变软,并且促进与果实花色苷积累相关的关键基因CHS和UFGT的转录水平。该研究有助于深入揭示果实发育信息传递的分子机制,也为今后草莓的分子改良育种奠定重要的基础。

关键词: 草莓, 果实, 脱落酸, 蔗糖, FaASR基因

Abstract:

In this study,we used Sweet Charlie strawberry as test materials,based on cloning the strawberries ASR homologous gene,we carried out the bioinformatics and temporal expression analysis of FaASR,by manipulating ASR gene expression level in strawberry fruit,we tested the changes of physiological indicators,including phenotypic changes,sugar,ABA,pigments content,and fruit firmness. In addition,we measured the expression changes of some anthocyanin-related gene,such as CHS and UFGT,by which we revealed the regulation mechanisms of ASR gene over strawberry fruit ripening. Strawberry ASR contained a typical domain of ABA/WDS that was related to fruit ripening and stress-resistance,and ASR gene over-expression could promote strawberry fruit coloring,endogenous ABA and sucrose accumulation,fruit softening,and induced the transcription levels of anthocyanin related genes CHS and UFGT. The present study will further reveal the molecular mechanisms of information transmission in fruit development,and will also lay an important foundation for future molecular improvement of strawberries breeding.

Key words: strawberry, fruit, ABA, sucrose, FaASR

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