Growth of Interspecific Hybrid Embryos between Brassica campestris ssp. pekinensis and B. oleracea var. acephala and Their in Vitro Culture

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (2): 501-503.

• 研究简报 • Previous Articles Next Articles

Growth of Interspecific Hybrid Embryos between Brassica campestris ssp. pekinensis and B. oleracea var. acephala and Their in Vitro Culture

ZHU Peng-fang¹,WEI Yu-tang²,and ZHANG Yue¹

(1Forestry College, 2Horticultural College, Shenyang Agricultural University, Shenyang, Liaoning 110161 China)

Received:2006-10-30 Revised:2007-01-22 Online:2007-04-25 Published:2007-04-25

Abstract

Abstract: There were obvious obstacles of interspecific hybrid embryo growth between CMS Brassica campestris ssp. Pekinensis × B. oleracea var. acephala. Embryos collapse began at sphere stage, with endosperm nuclei being surrounded by walls. Also, endosperms inflated extremely. However, heart embryos between B. campestris ssp. pekinensis×B. napus grew well, surrounding by endosperm nucleus. The available time of ovule rescue culture in vitro was from 6 to 17 days after pollination, and the best time of which was from 9 to 11 days. Surge culture in liquid media improved plantlets notably. Otherwise, flower subsidiaries and other nutritious additions had not shown remarkable influence.

Key words: Chinese cabbage, Kale, Embryo growth, in vitro

CLC Number:

ZHU Peng-fang;WEI Yu-tang;and ZHANG Yue. Growth of Interspecific Hybrid Embryos between Brassica campestris ssp. pekinensis and B. oleracea var. acephala and Their in Vitro Culture[J]. ACTA HORTICULTURAE SINICA, 2007, 34(2): 501-503.

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Growth of Interspecific Hybrid Embryos between Brassica campestris ssp. pekinensis and B. oleracea var. acephala and Their in Vitro Culture

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