Cloning and Functional Analysis of ERF Transcription Factor Gene PpERF1a in Peach

doi:10.16420/j.issn.0513-353x.2019-0872

ACTA HORTICULTURAE SINICA ›› 2020, Vol. 47 ›› Issue (6): 1165-1171.doi: 10.16420/j.issn.0513-353x.2019-0872

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Cloning and Functional Analysis of ERF Transcription Factor Gene PpERF1a in Peach

LIU Yong1,*，WANG Zeqiong1,2,*，GONG Linzhong1,**，WANG Furong1，WANG Huiliang1，AI Xiaoyan1，and HE Huaping1,**

1Institute of Fruit and Tea，Hubei Academy of Agricultural Sciences，Wuhan 430064，China；2Wuhan Institute of Bioengineering，Wuhan 430415，China

Online:2020-06-25 Published:2020-06-25

Abstract

Abstract: In this study，an ethylene response factor was cloned from‘Fengguang’nectarine and named as PpERF1a（ppa018178m）. The full length of PpERF1a was 600 bp，which encoded 199 amino acids. PpERF1a contained a single AP2 domain, which was consistent with the ERF families’ features. Subcellular localization assays showed that PpERF1a was targeted to nucleus and cell membrane. Fifteen transgenic Arabidopsis plants overexpressing PpERF1a were generated. Comparing with the wild type Arabidopsis plants，transgenic plants showed obvious stunted phenotype. Six transgenic plants kept on producing rosette leaves without bolting or producing seeds. Six transgenic plants bolted and flowered，but no seed. Three transgenic plants could flower and produce seed，but the seed yield of the transgenic plants was obviously lower than that of the wild type. These results showed that PpERF1a gene regulated the growth and development of transgenic Arabidopsis plants. This study provides a technical basis for further analysis the functions of PpERF1a gene in peach growth and development．

Key words: peach, ethylene response factor, subcellular localization, function analysis

CLC Number:

S 662.1

LIU Yong1,*，WANG Zeqiong1,2,*，GONG Linzhong1,**，WANG Furong1，WANG Huiliang1，AI Xiaoyan1，and HE Huaping1,**. Cloning and Functional Analysis of ERF Transcription Factor Gene PpERF1a in Peach[J]. ACTA HORTICULTURAE SINICA, 2020, 47(6): 1165-1171.

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Cloning and Functional Analysis of ERF Transcription Factor Gene PpERF1a in Peach

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