Functional Analysis of Longan TFL1 Homologous Genes by Over- expression in Arabidopsis thaliana and Tobacco

doi:10.16420/j.issn.0513-353x.2018-0571

ACTA HORTICULTURAE SINICA ›› 2019, Vol. 46 ›› Issue (10): 1936-1946.doi: 10.16420/j.issn.0513-353x.2018-0571

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Functional Analysis of Longan TFL1 Homologous Genes by Over- expression in Arabidopsis thaliana and Tobacco

ZHANG Yiyong，PENG Yuan，FU Zhiyuan，and ZENG Lihui*

College of Horticulture，Fujian Agriculture and Forestry University，Fuzhou 350002，China

Online:2019-10-25 Published:2019-10-25

Abstract

Abstract: In order to study the functions of longan TFL1 homologs DlTFL1-1 and DlTFL1-2，Plant expression vectors pCAMBIA2300-DlTFL1-1 and pCAMBIA2300-DlTFL1-2 were constructed and DlTFL1-1 and DlTFL1-2 were transformed into Arabidopsis and tobacco mediated by Agrobacterium tumefaciens GV3101. Effects of overexpression of DlTFL1-1 and DlTFL1-2 on flowering time and phenotypes of transgenic Arabidopsis and tobacco plants were observed. D1TFL1-1 and D1TFL1-2 proteins had a highly similar secondary structure with AtTFL1 and had a typical PEBP conserved domain，however，D1TFL1-1 and D1TFL1-1 proteins have significantly different protein tertiary structure with AtTFL1. The flowering time was delayed in both DlTFL1-1 and DlTFL1-2 transgenic Arabidopsis under both long-day and short-day conditions. Under long-day conditions，the flowering time of DlTFL1-1 and DlTFL1-2 transgenic Arabidopsis was delayed for 10.0 and 6.8 days，respectively. At the same time，DlTFL1 transgenic Arabidopsis plants had more branches in inflorescences. Under short-day conditions，the flowering time of DlTFL1-1 and DlTFL1-2 transgenic Arabidopsis was delayed for 5.3 and 3.0 days respectively. In addition，the delay of flowering time was also observed in DlTFL1-1 and DlTFL1-2 transgenic tobacco plants. qPCR results showed that under long-day conditions，the expression level of AtFT in leaves decreased significantly. The expression of AtLEAFY and AtAP1 in inflorescences were also significantly down-regulated. Our results suggested that DlTFL1-1 and DlTFL1-2 play an role in inhibiting floral bud differentiation，and they may be the flowering repressors in longan.

Key words: longan, TFL1 homologous gene, flowering repressing gene

CLC Number:

S 667.2

ZHANG Yiyong，PENG Yuan，FU Zhiyuan，and ZENG Lihui*. Functional Analysis of Longan TFL1 Homologous Genes by Over- expression in Arabidopsis thaliana and Tobacco[J]. ACTA HORTICULTURAE SINICA, 2019, 46(10): 1936-1946.

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Functional Analysis of Longan TFL1 Homologous Genes by Over- expression in Arabidopsis thaliana and Tobacco

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