Selection of Appropriate Reference Genes in Solanum aculeatissimum for Quantitative Gene Expression Studies Under Different Experimental Conditions

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (8): 1731-1738.

• Technologies and Methods • Previous Articles Next Articles

Selection of Appropriate Reference Genes in Solanum aculeatissimum for Quantitative Gene Expression Studies Under Different Experimental Conditions

ZHOU Xiao-hui，LIU Jun，and ZHUANG Yong*

（Jiangsu Key Laboratory for Horticultural Crop Genetic Improvement，Institute of Vegetable Crops，Jiangsu Academy of Agricultural Sciences，Nanjing 210014，China）

Received:2014-04-14 Online:2014-08-25 Published:2014-08-25

Abstract

Abstract: The present study aimed to select stable and optimal reference genes for gene expression analysis in Solanum aculeatissimum. A total of seven traditional reference genes including GAPDH，ACTIN，18sRNA，UBQ，TUA，EF1 and CYP were screened and compared by the real-time quantitative PCR. The mRNA levels of these candidate genes were evaluated under different experimental conditions，including different tissues，different hormone treatments，abiotic and biotic stress using three common statistical softwares，GeNorm，NormFinder and RefFinder. The data showed that TUA and 18sRNA were the most stable genes in different tissues. 18sRNA and ACTIN showed high stability in different hormone treatments sample pool. EF1 and GAPDH were the most stably expressed reference genes under salt and drought stress while TUA and EF1 exhibited the most stable expression across all of the tested S. aculeatissimum samples. CYP and ACTIN exhibited the least stable expression under most of the experimental conditions which are not recommended.

Key words: Solanum aculeatissimum, real-time quantitative PCR, reference gene

CLC Number:

S 641.1

ZHOU Xiao-hui，LIU Jun，and ZHUANG Yong*. Selection of Appropriate Reference Genes in Solanum aculeatissimum for Quantitative Gene Expression Studies Under Different Experimental Conditions[J]. ACTA HORTICULTURAE SINICA, 2014, 41(8): 1731-1738.

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Selection of Appropriate Reference Genes in Solanum aculeatissimum for Quantitative Gene Expression Studies Under Different Experimental Conditions

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