Studies on 16S rDNA Sequence of Citrus Huanglongbing Bacteria in China

ACTA HORTICULTURAE SINICA ›› 2008, Vol. 35 ›› Issue (5): 649-654.

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Studies on 16S rDNA Sequence of Citrus Huanglongbing Bacteria in China

DING Fang ^{1, 2, 3}, HONG Ni ^{1, 4}, ZHONG Yun ² , YI Gan-jun ^2* and WANG Guo-ping ^{1, 3*}

(¹College of Plant Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; ²Institute of Fruit Tree Research, Guangdong Academy of Agricultural Science, Guangzhou 510640, China; ³National Indoor Conservation Center of Virus-free Germplasm of Fruit Crops, Huazhong Agricultural University, Wuhan 430070, China; ⁴The Key Laboratory of Plant Pathology of Hubei Province, Huazhong Agricultural University, Wuhan 430070, China)

Received:2007-10-26 Revised:2008-04-14 Online:2008-05-25 Published:2008-05-25
Contact: YI Gan-jun

Abstract

Abstract:

PCR-RFLP-SSCP analysis was carried out to study the 16S rDNA of the citrus Huanglongbing (HLB) bacterial isolates collected from 7 provinces in China. Three restriction endonucleases were used to digest the target DNA fragment of 16S rDNA. The digested products were subjected to single-strand conformation polymorphism (SSCP) analysis. The results showed that there was no obvious difference among the 9 representative isolates from 7 provinces in 16S rDNA. The 16S rDNAs of the 9 representative isolates were cloned and sequenced. The result of the multiple alignment analysis of the sequences was in agreement with that of PCR-RFLP-SSCP analysis. It revealed that the citrus Huanglongbing bacteria isolated from different areas of China were highly conservative in their 16S rDNA sequence, and no molecular change were found among isolates from different hosts and different geographic areas. The result laid a foundation for further research on systematic evolution of HLB bacteria.

Key words: citrus, Huanglongbing, Candidatus, 16S rDNA, PCR-RFLP-SSCP, cloning, sequence analysis

CLC Number:

S 666

DING Fang;;;HONG Ni;;ZHONG Yun ;YI Gan-jun and WANG Guo-ping; . Studies on 16S rDNA Sequence of Citrus Huanglongbing Bacteria in China[J]. ACTA HORTICULTURAE SINICA, 2008, 35(5): 649-654.

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Studies on 16S rDNA Sequence of Citrus Huanglongbing Bacteria in China

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