Construction of Binary Expression Vector Containing Cry2Aa2 and PamPAP and Its Transfer to Pepper

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (7): 1285-.

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Construction of Binary Expression Vector Containing Cry2Aa2 and PamPAP and Its Transfer to Pepper

GAO Yu-Yao, CHEN Chang-Ming, CHEN Guo-Ju, CAO Bi-Hao, LEI Jian-Jun

College of Horticulture，South China Agricultural University，Guangzhou 510642，China；National Engineering Research Center for Space Breeding，Guangzhou 510642，China

Online:2012-07-25 Published:2012-07-25

Abstract

Abstract: The aim of this study was to obtain transgenic pepper plants with double resistances to virus and insect that could be used in developing new pepper variety. The non-virulent defective PAP gene （PamPAP），CaMV35S promoter and Nos terminal were inserted into MCS of pCAMBIA1301- Cry2Aa2-PMI. Cry2Aa2 and PamPAP were transferred into pepper（Capsicum annuum L.）mediated by Agrobacterium tumefaciens with PMI/Man selection system．The results of PCR and Southern blot indicated that Cry2Aa2 and PamPAP had been integrated into the pepper genome. RT-PCR analysis further showed that the Cry2Aa2 and PamPAP had been expressed simultaneously. In addition，resistances to virus and insect were assayed，and the results showed that the positive transgenic pepper plants had higher degree of resistances to CMV and Prodenia litura larva than the non-transgenic plants. This suggested that transgenic pepper plants with double resistances to virus and insect could be obtained by transferring Cry2Aa2 and PamPAP.

Key words: pepper, PMI/Man system, Cry2Aa2, PamPAP, genetic transformation, resistance to virus, resistance to insect

CLC Number:

S 641.3

GAO Yu-Yao, CHEN Chang-Ming, CHEN Guo-Ju, CAO Bi-Hao, LEI Jian-Jun. Construction of Binary Expression Vector Containing Cry2Aa2 and PamPAP and Its Transfer to Pepper[J]. ACTA HORTICULTURAE SINICA, 2012, 39(7): 1285-.

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Construction of Binary Expression Vector Containing Cry2Aa2 and PamPAP and Its Transfer to Pepper

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