Abstract: To explore the genetic variations in cucumber（Cucumis sativus L.，2n = 14）—sour cucumber（C. hystrix Chakr.，2n = 24）introgression lines，twelve introgression lines and their recipient parent cultivated cucumber‘Beijing Jietou’were used in analysis of biological traits derived from sour cucumber and detection of the relevant QTLs. The results indicated that coefficient of variation among different introgression lines ranged from 6.17% to 46.26%，with an average of 20.81%. And the majority of QTL traits had significant difference between recipient parent and 12 different introgression lines， showing the genetic characters of sour cucumber. By t test to P ≤ 0.001 as the threshold value，a total of 87 QTLs were detected in 10 traits，an average of 9.67 QTLs per trait，7.25 QTLs each introgression line and 0.8 QTL each introgressed DNA segment. Above results proved that the sour cucumber genetic material，which controlled related traits，had introgressed into cultivated cucumber，and the cucumber genetic basis had been expanded.

Key words: cucumber, introgression line, biological trait, genetic variation, QTL