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ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (4): 621-628.

• Fruit Trees • Previous Articles     Next Articles

Effects of DA-6 on Chlorophyll Biosythesis Pathway in Peach Leaves

 YANG  Qing, AI  Sha-Jiang- · Mai-Mai-Ti, WANG  Zhi-Xia, LIU  Guo-Jie   

  1. (Department of Pomology,College of Agronomy and Biotechnology,China Agricultural University,Beijing 100193,China)
  • Online:2012-04-25 Published:2012-04-25

Abstract: Taken ‘Xiangshan Honey’ peach as the material,the change of chlorophyll precursor contents and activity of the key enzymes in chlorophyll biosynthesis pathway after DA-6 (N-diethylaminoethyl hexanote)application were investigated the underlying mechanism of DA-6 induced leaf chlorophyll content increase. Compared with the control,20 mg · L-1 foliar DA-6 spray significantly increased the contents of chlorophylls,especially that of chlorophyll b,and chlorophyll intermediate products porphobilinogen(PBG)and uroporphyrinoge(Urogen Ⅲ),whereas decreased the amount of chlorophyll precursor 5-aminolevulinic acid(ALA). The results suggested the transformation from ALA to PBG and PBG to Urogen Ⅲ was up-regulated. The key enzymes catalyzing the two reactions are 5-aminolevulinate dehydratase(ALAD)and uroporporphyrinogn Ⅲ synthase(UROS),their enhanced activity after DA-6 foliar spraying was assayed. Our study demonstrated that foliar spray DA-6 increased the conversion efficiency of ALA to PBG and PBG to Urogen Ⅲ,up-regulated the chlorophyll biosynthesis pathway and resulted in higher chlorophyll content in peach leaves.

Key words: peach, DA-6, chlorophyll, biosynthesis, precursors, key enzyme, enzyme activity

CLC Number: