Effects of GMPase Overexpression on Ascorbic Acid Content and Relative Index to Low-temperature Tolerance in Tomato Plants

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (4): 692-700.

• Vegetables • Previous Articles Next Articles

Effects of GMPase Overexpression on Ascorbic Acid Content and Relative Index to Low-temperature Tolerance in Tomato Plants

LI Chao-han1，ZHANG Lin1，SHI Qing-hua1，LI Qing-zhu1，GUO Xiao-qing1，LI Xia1，and YU Xian-chang 2,*

¹ State Key Laboratory of Crop Biology，College of Horticulture Science and Engineering，Shandong Agricultural University，Tai’an，Shandong 271018，China；²Institute of Vegetables and Flowers，Chinese Academy of Agriculture Sciences，Beijing 100081，China

Received:2011-01-14 Revised:2011-03-09 Online:2011-04-25 Published:2011-04-25
Contact: YU Xian-chang

Abstract

Abstract: Two transgenic tomato plants of overexpressing GDP-D-mannose pyrophosphorylase gene （GMPase）were obtained by Agrobacterium-mediated transformation，and Western blot analysis indicated that the GMPase protein in transgenic plants was significantly increased. T0 make sure the function of GMPase overexpression，ascorbate content and cold tolerance capacity were studied. Compared with wild-type plants，the GMPase activity and contents of AsA and DHA in both normal and low-temperature conditions were increased and the MDA content was decreased in the transgenic plants. Based on these results，it can be concluded that the overexpression of GMPase may increase tomato tolerance to cold stress by increasing AsA level and lowering lipid peroxidation.

Key words: tomato, ascorbic acid, GDP-D-mannose pyrophosphorylase, transgenic, low-temperature stress

CLC Number:

S 641.2

LI Chao-han;ZHANG Lin;SHI Qing-hua;LI Qing-zhu;GUO Xiao-qing;LI Xia;and YU Xian-chang;. Effects of GMPase Overexpression on Ascorbic Acid Content and Relative Index to Low-temperature Tolerance in Tomato Plants [J]. ACTA HORTICULTURAE SINICA, 2011, 38(4): 692-700.

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Effects of GMPase Overexpression on Ascorbic Acid Content and Relative Index to Low-temperature Tolerance in Tomato Plants

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