Marker-assisted Selection of Near Isogenic Lines for Clubroot Resistant Gene in Chinese Cabbage

ACTA HORTICULTURAE SINICA ›› 2010, Vol. 37 ›› Issue (8): 1264-1272.

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Marker-assisted Selection of Near Isogenic Lines for Clubroot Resistant Gene in Chinese Cabbage

PIAO Zhong-yun*，WU Di，WANG Miao，and ZHANG Teng

（College of Horticulture，Shenyang Agricultural University，Shenyang 110866，China）

Received:2010-05-12 Revised:2010-06-30 Online:2010-08-25 Published:2010-08-25
Contact: PIAO Zhong-yun

Abstract

Abstract: Clubroot disease is one of the most serious diseases in Chinese cabbage. In this study，nine near isogenic lines（NILs）were developed from backcross of clubroot susceptible‘BJN3’× clubroot resistant‘CR Shinkii DH’line through marker-assisted selection（MAS）. Based on the foreground selection with TCR01 and TCR09 flanking the CRb gene and genomic background selection with 51 SSRs，10 homozygous resistant BC3F2 individuals recovering 98% of‘BJN3’genome were obtained. Results from inoculation of these BC3F2 individuals indicated that they were resistance to clubroot disease. By further selfing of BC3F2 individuals，nine BC3F3 individuals with full genome content of‘BJN3’were developed. Evaluation of heading related traits showed that there were no significant difference between the NILs and‘BJN3’.

Key words: Chinese cabbage, clubroot disease, CRb gene, marker-assisted selection, near isogenic line

CLC Number:

S 634.1

PIAO Zhong-yun;WU Di;WANG Miao;and ZHANG Teng. Marker-assisted Selection of Near Isogenic Lines for Clubroot Resistant Gene in Chinese Cabbage[J]. ACTA HORTICULTURAE SINICA, 2010, 37(8): 1264-1272.

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Marker-assisted Selection of Near Isogenic Lines for Clubroot Resistant Gene in Chinese Cabbage

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