Genetic Transformation of Carnation with Its ACC Oxida~ Antisense Gene

ACTA HORTICULTURAE SINICA ›› 2003, Vol. 30 ›› Issue (6): 699-702.

• 研究论文 • Previous Articles Next Articles

Genetic Transformation of Carnation with Its ACC Oxida~ Antisense Gene

Zhang Shuzhen ， Tang Huolong ， Yang Benpeng ，an d Liu Feihu。

( National研Biotechnology Laboratoryfor Tropical Crops，Chinese Academy ofTropic Agricultural Sciences，Haikou 571101，China；Co／／ege of Sc／ence，Yuamm Un／,~rshy，／(ammh~650091，China)

Received:2003-04-17 Revised:2003-06-11 Online:2003-12-25 Published:2003-12-25

Abstract

Abstract: The high frequency regeneration system of carnation from leaf to callus to plantlets was established．
Then genetic transformation of the carnation with the ACO an tisense gene．which controled by flower-specific expression
promoter(PchsA)was performed via agrobacterium mediated method and obtained 3 of transgenic plants
via the pathway of regeneration plantlets from callus at last．Th e resuhs of the PCR and PCR．Southem hybridization
analysis showed that the carnation ACC oxidase antisense gene Was integrated into carnation genomes．It will be a
good foundation for improvement the longevity of carnation flowers．

Key words: Carnation, ACC oxidase, Antisense gene, Genetic transformation

CLC Number:

s 68

Zhang Shuzhen;Tang Huolong;Yang Benpeng;an d Liu Feihu。. Genetic Transformation of Carnation with Its ACC Oxida~ Antisense Gene[J]. ACTA HORTICULTURAE SINICA, 2003, 30(6): 699-702.

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Genetic Transformation of Carnation with Its ACC Oxida~ Antisense Gene

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