Effects of Introduction of Different T-DNA Structures of ACO Gene to Tomato Genome on Ethylene Production Rate

ACTA HORTICULTURAE SINICA ›› 2007, Vol. 34 ›› Issue (3): 644-648.

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Effects of Introduction of Different T-DNA Structures of ACO Gene to Tomato Genome on Ethylene Production Rate

CHEN Yin-hua^1,3 ; L IHan-xia² ;YE Zhi-biao^1,2*

〔¹National Key Laboratory of Crop Genetic Improvem ent, Huazhong Agricultural University, Wuhan 430070, China; ²NationalCenter for Vegetable Improvement (Central China) , Huazhong Agricultural University, W uhan 430070, China; ³ Key Laborotoryfor Tropical Biological Resources, M inistry of Education, Haikou 570228, China〕

Received:1900-01-01 Revised:1900-01-01 Online:2007-06-25 Published:2007-06-25

Abstract

Abstract: To analyze the effect of different structures of foreign genes on the endogenous genes, three
different T2DNA structures of ACO gene were introduced into tomato genome through the method of Agrobac
turium 2mediated transformation. The ethylene evolution of the transgenic tomato p lants was measured by gas
chromatography. The results showed that in the three different transgenic p lants the maximum inhibition of en
dogenous ACO gene exp ression was observed using RNAi vectors.

Key words: Tomato, ACO gene, RNAi, Ethylene evolution

CHEN Yin-hua;;L IHan-xia;YE Zhi-biao;. Effects of Introduction of Different T-DNA Structures of ACO Gene to Tomato Genome on Ethylene Production Rate[J]. ACTA HORTICULTURAE SINICA, 2007, 34(3): 644-648.

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Effects of Introduction of Different T-DNA Structures of ACO Gene to Tomato Genome on Ethylene Production Rate

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