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Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (7): 1733-1744.doi: 10.16420/j.issn.0513-353x.2023-0624

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles     Next Articles

Cloning and Functional Analysis of Cuticular Wax Synthesis Gene SlCER1-7 in Tomato

ZHANG Ruiyi1, FENG Wenjia1, LI Benben1, SONG Yiying1, WEI Mingyue1, CHAI Guaiqiang1,2,*(), and HUO Yanbo1,2   

  1. 1 College of Advanced Agricultural Sciences,Yulin University,Yulin,Shaanxi 719000,China
    2 Shaanxi Key Laboratory of Ecological Restoration in Shaanbei Mining Area,Yulin,Shaanxi 719000,China
  • Received:2025-04-01 Revised:2025-05-21 Online:2025-07-23 Published:2025-07-23
  • Contact: CHAI Guaiqiang

Abstract:

The bioinformatics,expression analysis and transgenic technology were used to reveal the biological function of SlCER1-7,the results showed that SlCER1-7 had a total length of 2 078 bp and contained a complete open reading frame of 1 884 bp,encoding 627 amino acids. Bioinformatics analysis showed that SlCER1-7 was a hydrophilic protein. Homology alignment and phylogenetic analysis showed that SlCER1-7 was closely related to CER1 in eggplant. Meanwhile,SLCER1-7 was mainly expressed in mature leaves,green pericarp,yellow pericarp and red pericarp,but not in young leaves. Finally,the overexpression transgenic lines were obtained by Agrobacterium-mediated transformation,the results showed that the total wax content of transgenic tomato fruits were significantly increased,especially the straight chain C33 alkanes and the branched chain C32 alkanes,and the water-holding capacity of transgenic fruits were enhanced.

Key words: tomato, cuticular wax, CER1, gene cloning, water retention