Establishment and Optimization of Regeneration System for Apple Rootstock B9

doi:10.16420/j.issn.0513-353x.2024-0707

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (4): 947-958.doi: 10.16420/j.issn.0513-353x.2024-0707

• Cultivation·Physiology & Biochemistry • Previous Articles Next Articles

Establishment and Optimization of Regeneration System for Apple Rootstock B9

TIAN Yufeng, MA Songya^*(), YANG An, HAN Xiaolei, ZHANG Caixia^*()

National Apple Breeding Center，Key Laboratory of Horticultural Crop Germplasm Resource Utilization of the Ministry of Agriculture and Rural Affairs，Institute of Pomology Research，Chinese Academy of Agricultural Sciences，Xingcheng，Liaoning 125100，China

Received:2024-11-08 Revised:2025-01-17 Online:2025-05-08 Published:2025-04-25
Contact: MA Songya, ZHANG Caixia

Abstract

Abstract:

To establish an efficient regeneration system for apple rootstock，this study utilized detached in vitro leaves from tissue-cultured seedlings of apple rootstock B9 as explants. This study screened key factors influencing regeneration，including various concentrations of phytohormone combinations，durations of dark culture，seedling ages，and hygromycin concentration. The findings revealed that the optimal medium for inducing indefinite buds was MS + 3.0 mg · L^-1 TDZ + 0.3 mg · L^-1 NAA + 30 g · L^-1 sucrose + 7 g · L^-1 agar，with a seedling age of 45 days and a dark culture period of 14 days，leading to the highest regeneration efficiency. The proliferation medium consisted of MS + 0.8 mg · L^-1 6-BA + 0.2 mg · L^-1 IAA + 30 g·L^-1 sucrose + 7 g · L^-1 agar；The rooting medium was 1/2 MS + 0.6 mg · L^-1 IBA + 30 g · L^-1 sucrose + 7 g · L^-1 agar. The appropriate hygromycin concentration for leaf regeneration was 2-4 mg · L^-1，while for rooting，it was 2-3 mg · L^-1.

Key words: apple, rootstock, regeneration, phytohormone, indefinite buds

TIAN Yufeng, MA Songya, YANG An, HAN Xiaolei, ZHANG Caixia. Establishment and Optimization of Regeneration System for Apple Rootstock B9[J]. Acta Horticulturae Sinica, 2025, 52(4): 947-958.

Figures/Tables 12

References 41

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TDZ/（mg · L^-1）		再生效率/% Regeneration efficiency	平均再生芽数/个 Average number of regenerated buds
1	0.1	0.83 ± 0.42 e	1.67 ± 0.88 b
1	0.3	22.08 ± 1.10 d	1.61 ± 0.07 b
1	0.5	16.67 ± 2.20 d	1.30 ± 0.03 b
2	0.1	26.25 ± 1.44 d	1.84 ± 0.02 b
2	0.3	44.58 ± 4.41 c	2.10 ± 0.10 ab
2	0.5	52.08 ± 4.70 bc	2.20 ± 0.04 ab
3	0.1	63.33 ± 6.86 bc	2.20 ± 0.11 ab
3	0.3	80.42 ± 7.95 a	2.93 ± 0.13 a
3	0.5	45.83 ± 4.23 c	1.94 ± 0.05 b

TDZ/（mg · L^-1）		再生效率/% Regeneration efficiency	平均再生芽数/个 Average number of regenerated buds
1	0.1	0.83 ± 0.42 e	1.67 ± 0.88 b
1	0.3	22.08 ± 1.10 d	1.61 ± 0.07 b
1	0.5	16.67 ± 2.20 d	1.30 ± 0.03 b
2	0.1	26.25 ± 1.44 d	1.84 ± 0.02 b
2	0.3	44.58 ± 4.41 c	2.10 ± 0.10 ab
2	0.5	52.08 ± 4.70 bc	2.20 ± 0.04 ab
3	0.1	63.33 ± 6.86 bc	2.20 ± 0.11 ab
3	0.3	80.42 ± 7.95 a	2.93 ± 0.13 a
3	0.5	45.83 ± 4.23 c	1.94 ± 0.05 b

暗培养时间/d Dark incubation time	调查个数 Number of investigations	再生效率/% Regeneration efficiency	平均再生芽数/个 Average number of regenerated buds
7	80	37.50 ± 4.73 b	1.91 ± 0.16 a
14	80	66.25 ± 5.77 a	2.38 ± 0.07 a
21	80	48.33 ± 6.05 ab	2.36 ± 0.11 a

暗培养时间/d Dark incubation time	调查个数 Number of investigations	再生效率/% Regeneration efficiency	平均再生芽数/个 Average number of regenerated buds
7	80	37.50 ± 4.73 b	1.91 ± 0.16 a
14	80	66.25 ± 5.77 a	2.38 ± 0.07 a
21	80	48.33 ± 6.05 ab	2.36 ± 0.11 a

苗龄/d Seedling age	数量 Number	再生效率/% Regeneration efficiency	平均再生芽数/个 Average number of regenerated buds
35	80	35.00 ± 3.31 b	1.80 ± 0.58 a
45	80	69.58 ± 3.97 a	2.58 ± 0.17 a
55	80	40.42 ± 4.10 b	1.92 ± 0.22 a

Establishment and Optimization of Regeneration System for Apple Rootstock B9

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6-BA/（mg · L^-1）	IAA/（mg · L^-1）	GA₃/（mg · L^-1）	增殖倍数 Multiplication multiple
0.4	0.1	0.2	1.13 ± 0.14 d
0.8	0.1	0.2	2.11 ± 0.11 bc
1.2	0.1	0.2	4.45 ± 0.16 a
0.4	0.2	0.2	2.89 ± 0.16 b
0.8	0.2	0.2	3.75 ± 0.21 a
1.2	0.2	0.2	1.55 ± 0.16 cd

IBA /（mg · L^-1）	生根率/% Rooting rate	平均生根数 Average rooting number
0.2	21.11 ± 4.01 d	2.51 ± 0.24 b
0.4	40.00 ± 5.77 cd	2.66 ± 0.12 b
0.6	81.11 ± 4.84 a	5.99 ± 0.30 a
0.8	66.67 ± 5.09 ab	5.18 ± 0.72 a
1.0	52.22 ± 2.94 bc	2.77 ± 0.09 b
1.2	23.33 ± 5.09 d	3.17 ± 0.28 b

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