Genetic Diversity Analysis and SSR Fingerprint Construction of Evergreen Rhododendron Germplasms

doi:10.16420/j.issn.0513-353x.2024-0158

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (2): 380-394.doi: 10.16420/j.issn.0513-353x.2024-0158

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Genetic Diversity Analysis and SSR Fingerprint Construction of Evergreen Rhododendron Germplasms

ZHANG Lu¹, ZHANG Pingping², XIE Weijia¹, XU Feng¹, PENG Lüchun¹, SONG Jie¹, DU Guanghui²^,^*(), LI Shifeng¹^,^*()

¹ National Engineering Research Center for Ornamental Horticulture，Key Laboratory of Flower Breeding of Yunnan Province，Flower Research Institute of Yunnan Academy of Agricultural Sciencese，Kunming 650205，China
² Resource Plant Research Institute，Yunnan University，Kunming 650504，China

Received:2024-05-22 Revised:2024-11-07 Online:2025-02-25 Published:2025-02-23
Contact: DU Guanghui, LI Shifeng

Abstract

Abstract:

In this study，based on genomic sequencing data of Rhododendron delavayi Franch.，genomic-SSR distribution characteristics were analyzed and polymorphic SSR primers were developed. Furthermore，the 61 evergreen Rhododendron germplasms were analyzed for genetic relationship and fingerprint construction to realize their genetic relationships and speculate their possible parents. In the results，15 pairs of SSR primers with high polymorphism，good stability and strong intergeneric versatility were developed，and a total of 126 alleles were detected in 61 evergreen Rhododendron germplasms. The average number of alleles（N_a）and the average effective number of alleles（N_e）were 8.400 and 3.4681，respectively. The mean observed heterozygosity（H_o）and expected heterozygosity（H_e）were 0.3421 and 0.5347，respectively，and the mean polymorphism information content（PIC）was 0.5163. According to the results of population genetic structure analysis，61 evergreen Rhododendron germplasms were divided into 3 subgroups，which were basically consistent with the results of phylogenetic analysis. Combined with the pedigree information，it was inferred that the ancestral parents of the introduced Japanese cultivars came from two different distribution areas，and all cultivars，including the self-bred cultivars，were the hybrids of the parental species from sect. Ponticum in subgen. Hymenanthes. Five SSR markers with high discriminability were used to construct the fingerprint of 61 evergreen Rhododendron germplasms successfully.

Key words: evergreen rhododendron, genomic SSR markers, genetic diversity, fingerprints

ZHANG Lu, ZHANG Pingping, XIE Weijia, XU Feng, PENG Lüchun, SONG Jie, DU Guanghui, LI Shifeng. Genetic Diversity Analysis and SSR Fingerprint Construction of Evergreen Rhododendron Germplasms[J]. Acta Horticulturae Sinica, 2025, 52(2): 380-394.

Figures/Tables 9

References 36

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序号 No.	编号 ID	种/品种名 Species/cultivars name	分类/谱系（♀ × ♂） Classification/pedigree
1	WS-1	大喇叭杜鹃R. excellens	杜鹃亚属杜鹃组有鳞大花亚组subgen. Rhododendron sect. Rhododendron subsect. Maddenia
2	WS-3	露珠杜鹃R. irroratum	常绿杜鹃亚属常绿杜鹃组露珠杜鹃亚组subgen. Hymenanthes sect. Ponticum subsect. Irrorata
3	WS-4	大白杜鹃R. decorum	常绿杜鹃亚属常绿杜鹃组云锦杜鹃亚组subgen. Hymenanthes sect. Ponticum subsect. Fortunea
4	WS-5	滇南杜鹃R. hancockii	马银花亚属长蕊组subgen. Azaleastrum sect. Choniastrum
5	WS-6	云南杜鹃R. yunnanense	杜鹃亚属杜鹃组三花杜鹃亚组subgen. Rhododendron sect. Rhododendron subsect.Triflora
6	WS-7	宽杯杜鹃R. sinofalconeri	常绿杜鹃亚属常绿杜鹃组杯毛杜鹃亚组 subgen. Hymenanthes sect. Ponticum subsect. Falconera
7	WS-8	马缨杜鹃R. delavayi	常绿杜鹃亚属常绿杜鹃组树形杜鹃亚组subgen. Hymenanthes sect. Ponticum subsect.Arborea
8	WS-9	马银花杜鹃R. ovatum	马银花亚属马银花组subgen. Azaleastrum sect. Azaleastrum
9	W6	凯特Catawbiense Grandiflorum	Catawbiense × 不详Unknown
10	W13	伊优克 Eucharitis	不详Unknown
11	W14	闪烁 Goldflimmer	catawbiense or Ponticum seedling
12	W17	莱姆 Halfdan Lem	The Hon. Jean Marie de Montague × Red Loderi
13	W19	门铃 Jingle Bells	Fabia group × Ole Olson
14	W21	罗伯茨 Lord Roberts	Catawbiense × 不详Unknown
15	W27	红杰克 Red Jack	Wilgen’s Ruby × May Day
16	W29	星光 Scintillation	不详Unknown
17	W31	玳瑁橙 Tortoiseshell Orange	Tortoiseshell group
18	W33	红珍珠 Wilgen’s Ruby	Britannia × John Walter
19	W35	锦缎 Germania	Antoon van Welie × Williamsianum
20	R1	诺娃 Nova Zembla	Parsons Grandiflorum × dark red hybrid
21	R2	粉黛 XXL	President Roosevelt × W35
22	J1	普罗米修斯 Prominence	不详Unknown
23	J2	紫色彩虹 Purple Rainbow	不详Unknown
24	J3	紫色宫殿 Purple Dome	不详Unknown
25	J4	红蝶 Red Monarch	不详Unknown
26	J5	薰衣草王子 Lavendor Prince	不详Unknown
27	J6	草莓圣代 Strawberry Sundae	不详Unknown
28	J7	珍珠姬 Sin Juhime	不详Unknown
29	J8	玛丽亚 Marian	不详Unknown
30	J9	甜心 Sweet Heart	不详Unknown
31	J10	阿特拉斯 Atlas	不详Unknown
32	J11	藤井夫人 Mrs Fujii	不详Unknown
33	J13	婚礼花束 Wedding Bouquet	不详Unknown
34	J14	太阳 Sun	不详Unknown
35	J16	彩虹 Rainbow	不详Unknown
36	J18	雨滴 Raindrop	不详Unknown
37	J19	火把 Torch	不详Unknown
38	J20	大地 Earth	不详Unknown
39	ZY-1	金色铃铛 Jinse Lingdang	W19 × 大白杜鹃 W19 × R. decorum
40	ZY-2	贵妃醉酒 Guifei Zuijiu	W13 × 大白杜鹃 W13 × R. decorum
41	ZY-3	云之绯羽 Yunzhifeiyu	W29 × 露珠杜鹃 W29 × R. irroratum
42	ZY-4	云之染彩 Yunzhirancai	R1 × 大白杜鹃 R1 × R. decorum
43	ZY-5	翠湖晓妆 Cuihu Xiaozhuang	W6 × R2
44	ZY-6	翠湖怜香 Cuihu Lianxiang	W21 × 大白杜鹃 W21 × R. decorum
45	ZY-7	红妆 Hongzhuang	W35 × R2
46	ZY-8	芳华 Fanghua	W35 × R2
47	ZY-9	黛玉 Daiyu	W35 × R2
48	ZY-10	花妍 Huayan	W35 × R2
49	ZY-11	墨碟心 Modiexin	（Ponticum × Unknown）
50	ZY-12	粉精灵 Fenjingling	大白杜鹃芽变 Bud sport of R. decorum
51	ZY-13	云之蜜语 Yunzhimiyu	W13 × 大白杜鹃 W13 × R. decorum
52	ZY-14	阳春雪 Yangchunxue	W35 × R2
53	ZY-15	宝钗 Baochai	大白杜鹃 × R2 R. decorum × R2
54	ZY-16	湘云 Xiangyun	R2 × 露珠杜鹃 R2 × R. irroratum
55	ZY-17	怡红 Yihong	R2 × 马缨杜鹃 R2 × R. delavayi
56	ZY-18	落雁 Luoyan	（Ponticum × Unknown）
57	ZY-19	昆上初红 Kunshang Chuhong	大白杜鹃 × 马缨杜鹃 R. decorum × R. delavayi
58	ZY-20	罗裳 Luochang	W29 × 马塞尔（Ponticum × Unknown）
59	ZY-21	翠湖紫鸥 Cuihu Ziou	安娜（Ponticum × Unknown）× R1
60	ZY-22	翠湖彤云 Cuihu Tongyun	安娜（Ponticum × Unknown）× R1
61	ZY-23	翠湖涟漪 Cuihu Lianyi	大白杜鹃 × W6 R. decorum × W6

序号 No.	编号 ID	种/品种名 Species/cultivars name	分类/谱系（♀ × ♂） Classification/pedigree
1	WS-1	大喇叭杜鹃R. excellens	杜鹃亚属杜鹃组有鳞大花亚组subgen. Rhododendron sect. Rhododendron subsect. Maddenia
2	WS-3	露珠杜鹃R. irroratum	常绿杜鹃亚属常绿杜鹃组露珠杜鹃亚组subgen. Hymenanthes sect. Ponticum subsect. Irrorata
3	WS-4	大白杜鹃R. decorum	常绿杜鹃亚属常绿杜鹃组云锦杜鹃亚组subgen. Hymenanthes sect. Ponticum subsect. Fortunea
4	WS-5	滇南杜鹃R. hancockii	马银花亚属长蕊组subgen. Azaleastrum sect. Choniastrum
5	WS-6	云南杜鹃R. yunnanense	杜鹃亚属杜鹃组三花杜鹃亚组subgen. Rhododendron sect. Rhododendron subsect.Triflora
6	WS-7	宽杯杜鹃R. sinofalconeri	常绿杜鹃亚属常绿杜鹃组杯毛杜鹃亚组 subgen. Hymenanthes sect. Ponticum subsect. Falconera
7	WS-8	马缨杜鹃R. delavayi	常绿杜鹃亚属常绿杜鹃组树形杜鹃亚组subgen. Hymenanthes sect. Ponticum subsect.Arborea
8	WS-9	马银花杜鹃R. ovatum	马银花亚属马银花组subgen. Azaleastrum sect. Azaleastrum
9	W6	凯特Catawbiense Grandiflorum	Catawbiense × 不详Unknown
10	W13	伊优克 Eucharitis	不详Unknown
11	W14	闪烁 Goldflimmer	catawbiense or Ponticum seedling
12	W17	莱姆 Halfdan Lem	The Hon. Jean Marie de Montague × Red Loderi
13	W19	门铃 Jingle Bells	Fabia group × Ole Olson
14	W21	罗伯茨 Lord Roberts	Catawbiense × 不详Unknown
15	W27	红杰克 Red Jack	Wilgen’s Ruby × May Day
16	W29	星光 Scintillation	不详Unknown
17	W31	玳瑁橙 Tortoiseshell Orange	Tortoiseshell group
18	W33	红珍珠 Wilgen’s Ruby	Britannia × John Walter
19	W35	锦缎 Germania	Antoon van Welie × Williamsianum
20	R1	诺娃 Nova Zembla	Parsons Grandiflorum × dark red hybrid
21	R2	粉黛 XXL	President Roosevelt × W35
22	J1	普罗米修斯 Prominence	不详Unknown
23	J2	紫色彩虹 Purple Rainbow	不详Unknown
24	J3	紫色宫殿 Purple Dome	不详Unknown
25	J4	红蝶 Red Monarch	不详Unknown
26	J5	薰衣草王子 Lavendor Prince	不详Unknown
27	J6	草莓圣代 Strawberry Sundae	不详Unknown
28	J7	珍珠姬 Sin Juhime	不详Unknown
29	J8	玛丽亚 Marian	不详Unknown
30	J9	甜心 Sweet Heart	不详Unknown
31	J10	阿特拉斯 Atlas	不详Unknown
32	J11	藤井夫人 Mrs Fujii	不详Unknown
33	J13	婚礼花束 Wedding Bouquet	不详Unknown
34	J14	太阳 Sun	不详Unknown
35	J16	彩虹 Rainbow	不详Unknown
36	J18	雨滴 Raindrop	不详Unknown
37	J19	火把 Torch	不详Unknown
38	J20	大地 Earth	不详Unknown
39	ZY-1	金色铃铛 Jinse Lingdang	W19 × 大白杜鹃 W19 × R. decorum
40	ZY-2	贵妃醉酒 Guifei Zuijiu	W13 × 大白杜鹃 W13 × R. decorum
41	ZY-3	云之绯羽 Yunzhifeiyu	W29 × 露珠杜鹃 W29 × R. irroratum
42	ZY-4	云之染彩 Yunzhirancai	R1 × 大白杜鹃 R1 × R. decorum
43	ZY-5	翠湖晓妆 Cuihu Xiaozhuang	W6 × R2
44	ZY-6	翠湖怜香 Cuihu Lianxiang	W21 × 大白杜鹃 W21 × R. decorum
45	ZY-7	红妆 Hongzhuang	W35 × R2
46	ZY-8	芳华 Fanghua	W35 × R2
47	ZY-9	黛玉 Daiyu	W35 × R2
48	ZY-10	花妍 Huayan	W35 × R2
49	ZY-11	墨碟心 Modiexin	（Ponticum × Unknown）
50	ZY-12	粉精灵 Fenjingling	大白杜鹃芽变 Bud sport of R. decorum
51	ZY-13	云之蜜语 Yunzhimiyu	W13 × 大白杜鹃 W13 × R. decorum
52	ZY-14	阳春雪 Yangchunxue	W35 × R2
53	ZY-15	宝钗 Baochai	大白杜鹃 × R2 R. decorum × R2
54	ZY-16	湘云 Xiangyun	R2 × 露珠杜鹃 R2 × R. irroratum
55	ZY-17	怡红 Yihong	R2 × 马缨杜鹃 R2 × R. delavayi
56	ZY-18	落雁 Luoyan	（Ponticum × Unknown）
57	ZY-19	昆上初红 Kunshang Chuhong	大白杜鹃 × 马缨杜鹃 R. decorum × R. delavayi
58	ZY-20	罗裳 Luochang	W29 × 马塞尔（Ponticum × Unknown）
59	ZY-21	翠湖紫鸥 Cuihu Ziou	安娜（Ponticum × Unknown）× R1
60	ZY-22	翠湖彤云 Cuihu Tongyun	安娜（Ponticum × Unknown）× R1
61	ZY-23	翠湖涟漪 Cuihu Lianyi	大白杜鹃 × W6 R. decorum × W6

SSR类型 SSR type	类型数 Number	重复次数 Repeats	占比/% Ratios	重复基序 Repeat motifs	SSR数量 Number of SSR	总占比/% Total ratios
单核苷酸 Mono-nucleotide	2	≥ 10	35.19	A/T C/G	41 699 913	34.01 0.74
二核苷酸 Di-nucleotide	6	≥ 6	41.63	AA/TT AC/GT AG/CT AT/AT CC/GG CG/CG	16 900 4 444 24 151 4 448 361 109	13.78 3.62 19.70 3.63 0.29 0.09
三核苷酸 Tri-nucleotide	12	≥ 5	10.23	AAA/TTT AAC/GTT AAG/CTT AAT/ATT ACC/GGT 其他 Others	5 260 582 1 786 630 1 523 4 131	4.29 0.47 1.46 0.51 1.24 3.37
四核苷酸 Tetra-nuleotide	33	≥ 5	9.47	AAAA/TTTT AAAC/GTTT AAAG/CTTT AAAT/ATTT 其他 Others	368 36 77 159 10 828	0.30 0.03 0.06 0.13 8.83
五核苷酸 Penta-nucleotide	39	≥ 5	0.20	AAAAG/CTTTT 其他 Others	48 203	0.04 0.17
六核苷酸 Hexa-nucleotide	85	≥ 5	3.28	AAAAAG/CTTTTT 其他 Others	13 3 956	0.01 3.23
合计Total	177		100.00		122 625	100.00

SSR类型 SSR type	类型数 Number	重复次数 Repeats	占比/% Ratios	重复基序 Repeat motifs	SSR数量 Number of SSR	总占比/% Total ratios
单核苷酸 Mono-nucleotide	2	≥ 10	35.19	A/T C/G	41 699 913	34.01 0.74
二核苷酸 Di-nucleotide	6	≥ 6	41.63	AA/TT AC/GT AG/CT AT/AT CC/GG CG/CG	16 900 4 444 24 151 4 448 361 109	13.78 3.62 19.70 3.63 0.29 0.09
三核苷酸 Tri-nucleotide	12	≥ 5	10.23	AAA/TTT AAC/GTT AAG/CTT AAT/ATT ACC/GGT 其他 Others	5 260 582 1 786 630 1 523 4 131	4.29 0.47 1.46 0.51 1.24 3.37
四核苷酸 Tetra-nuleotide	33	≥ 5	9.47	AAAA/TTTT AAAC/GTTT AAAG/CTTT AAAT/ATTT 其他 Others	368 36 77 159 10 828	0.30 0.03 0.06 0.13 8.83
五核苷酸 Penta-nucleotide	39	≥ 5	0.20	AAAAG/CTTTT 其他 Others	48 203	0.04 0.17
六核苷酸 Hexa-nucleotide	85	≥ 5	3.28	AAAAAG/CTTTTT 其他 Others	13 3 956	0.01 3.23
合计Total	177		100.00		122 625	100.00

编号 Code	位点 Loci	染色体号 Chr.	重复单元 Repeat unit	荧光标记 Fluorescent dye	引物序列（5′-3′） Primer Sequences	产物/bp Product	PIC
RdSSR01	Rhdel01G0240300	1	(AAA)₅	FAM	F：CTCTCAAATATTCGCCAGCC； R：CCTCTTCTGTCGGGTGAAAC	113 ~ 116	0.6132
RdSSR02	Rhdel03G0011900	3	(CGG)₆	FAM	F：ACTGGCGTTTGGAATTTGAC； R：TCCAAAGCAATCTTCCATCC	199 ~ 221	0.4730
RdSSR03	Rhdel03G0235100	3	(CGG)₅	HEX	F：CACGAGATTGGGAGTACGGT； R：AGCTGCGAGTATCCCAGTGT	278 ~ 287	0.8942
RdSSR04	Rhdel03G0250000	3	(AAG)₅	HEX	F：GCGTTACCGGCAGTATTGTT； R：GTGGCCTCTAGCCTTGTGAG	207 ~ 241	0.6773
RdSSR05	Rhdel03G0272700	3	(CTT)₅	FAM	F：CAACCAAGCAAGTGCAAAGA； R：CTGAGGCCTGTCTGGAAGTC	235 ~ 257	0.6111
RdSSR06	Rhdel04G0032400	4	(GCA)₇	HEX	F：GAATTCTTTCGCCACTCTGC； R：AAGCCATGGAGTTGATACGG	128 ~ 144	0.7279
RdSSR07	Rhdel04G0036900	4	(CCG)₆	HEX	F：ACGCCGCTAATAACCACAAC； R：GATAAATCCGGTGAATCCGA	224 ~ 240	0.5445
RdSSR08	Rhdel04G0139400	4	(GA)₈	HEX	F：CTCACGTGGTCCATCATTTG； R：TAGGGTTCCATCACAAAGCC	291 ~ 307	0.8265
RdSSR09	Rhdel04G0358500	4	(TC)₉	HEX	F：TGTGGAACATCTTGAGCAGC； R：AGTCAACAGGCCAAACCAAC	127 ~ 204	0.7858
RdSSR10	Rhdel05G0013300	5	(AG)₉	HEX	F：GGTGCACCAAGGTCTTCATT； R：ATCTATACCCGCTGCAATCG	281 ~ 288	0.6934
RdSSR11	Rhdel05G0137700	5	(GGA)₆	HEX	F：CATGCTGAAGAGTTGGACGA； R：CTTTGGAGACTGGAGGCTTG	151 ~ 155	0.2392
RdSSR12	Rhdel08G0018000	8	(AG)₈	FAM	F：GCTATGGAGTGCTTTAGCCG； R：TGATTGCTACGATCGCTTTG	131 ~ 150	0.4846
RdSSR13	Rhdel12G0119900	12	(AAC)₉	TAM	F：TCATCGACATCGTCAGCTTC； R：GCCTGATTTCACCTCGTGAT	227 ~ 245	0.7038
RdSSR14	Rhdel12G0190300	12	(TTC)₇	FAM	F：GGAGCAGCAGAACAATGAC； R：GTAAAGGGCGTTCGTATGGA	189 ~ 339	0.8926
RdSSR15	Rhdel13G0101600	13	(TC)₈	FAM	F：CCATGGAAATTGATTGGACC； R：ACAAATGGGCTAACTGGACG	184 ~ 296	0.3402
平均 Average							0.6338

Genetic Diversity Analysis and SSR Fingerprint Construction of Evergreen Rhododendron Germplasms

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编号 Code No.	SSR位点 SSR loci	等位基因数 N_a	有效等位基因数 N_e	观测杂合度 H_o	期望杂合度 H_e	多态性信息量 PIC	鉴定概率 PI	组合后鉴定概率 PI_sibs
RdSSR01	Rhdel01G0240300	3	1.4854	0.1639	0.3268	0.2907	0.4893	0.7089
RdSSR02	Rhdel03G0011900	5	1.1633	0.0328	0.1404	0.1375	0.7418	0.8652
RdSSR03	Rhdel03G0235100	5	2.4153	0.1887	0.5860	0.6100	0.2579	0.5215
RdSSR04	Rhdel03G0250000	25	14.7366	0.8197	0.9321	0.9280	0.0087	0.2861
RdSSR05	Rhdel03G0272700	4	1.8015	0.4590	0.4449	0.4081	0.3450	0.6137
RdSSR06	Rhdel04G0032400	8	3.7099	0.7213	0.7304	0.6984	0.1047	0.4109
RdSSR07	Rhdel04G0036900	6	1.9731	0.2000	0.4932	0.4834	0.2844	0.5745
RdSSR08	Rhdel04G0139400	9	2.3600	0.1525	0.5763	0.5676	0.2184	0.5165
RdSSR09	Rhdel04G0358500	9	3.2316	0.0667	0.6906	0.6542	0.1449	0.4409
RdSSR10	Rhdel05G0013300	3	1.8237	0.3333	0.4517	0.3976	0.3769	0.6183
RdSSR11	Rhdel05G0137700	2	1.2550	0.1311	0.2032	0.1825	0.6556	0.8123
RdSSR12	Rhdel08G0018000	5	1.2927	0.1475	0.2264	0.2197	0.6052	0.7881
RdSSR13	Rhdel12G0119900	8	2.9380	0.4262	0.6596	0.6020	0.1735	0.4635
RdSSR14	Rhdel12G0190300	24	8.7863	0.3934	0.8862	0.8771	0.0221	0.3124
RdSSR15	Rhdel13G0101600	10	3.0493	0.8947	0.6721	0.6879	0.1325	0.4471
均值Mean		8.400	3.4681	0.3421	0.5347	0.5163	0.3040	0.5587

编号 ID	种质名 Name	SSR指纹识别码 SSR fingerprint code	编号 ID	种质名 Name	SSR指纹识别码 SSR fingerprint code
WS-1	大喇叭杜鹃 R. excellens	A223-232B269-271C132-137D115E287	J11	藤井夫人 Mrs Fujii	A222B289C132-141D113-115E285
WS-3	露珠杜鹃 R. irroratum	A218-220B295C132-137D115E282	J13	婚礼花束 Wedding Bouquet	A228-239B287C132-137D115E285
WS-4	大白杜鹃 R. decorum	A216-233B280C135D115E285	J14	太阳 Sun	A224-231B275C137-141D113E285
WS-5	滇南杜鹃 R. hancockii	A247-253B309-338C132D115E282	J16	彩虹 Rainbow	A227-228B287C137-141D115E282
WS-6	云南杜鹃 R. yunnanense	A215-219B286-329C128-137D113E285	J18	雨滴 Raindrop	A222-228B275-287C132-135D115E282-287
WS-7	宽杯杜鹃 R. sinofalconeri	A210-228B302C144D115E282-285	J19	火把 Torch	A227B275C132-137D115E285
WS-8	马缨杜鹃 R. delavayi	A218-220B275C135D115E282-287	J20	大地 Earth	A228-231B287-289C132-137D115E282-285
WS-9	马银花 R. ovatum	A218B190C132-137D113-115	ZY-1	金色铃铛 Jinse Lingdang	A220-224B286C139D115E282-285
W6	凯特Catawbiense Grandiflorum	A220-239B297-315C135-137D115E285	ZY-2	贵妃醉酒 Guifei Zuijiu	A227B301C128-137D115E285
W13	伊优克 Eucharitis	A207-227B289-301C128-137D115E282- 285	ZY-3	云之绯羽 Yunzhifeiyu	A235-237B287-289C139D115E282
W14	闪烁 Goldflimmer	A224-239B307-313C137D112-115E285	ZY-4	云之染彩 Yunzhi Rancai	A235-239B289C132-137D115E282
W17	莱姆 Halfdan Lem	A220B275C132-137D115E285	ZY-5	翠湖晓妆 Cuihu Xiaozhuang	A212-214B291C132-135D115E285
W19	门铃 Jingle Bells	A224-231B283-286C147D115E285	ZY-6	翠湖怜香 Cuihu Lianxiang	A228-243B275C137-141D115E282-285
W21	罗伯茨 Lord Roberts	A228B275C137D115E282	ZY-7	红妆 Hongzhuang	A214-218B313-347C132-137D115E285
W27	红杰克 Red Jack	A239B275-283C137D115E282-285	ZY-8	芳华 Fanghua	A214-218B313-331C132-137D115E285
W29	星光 Scintillation	A239-263B289C132-137D115E285	ZY-9	黛玉 Daiyu	A207-218B301-313C132-137D115E285
W31	玳瑁橙 Tortoiseshell Orange	A220-237B286-299C135-147D115E285	ZY-10	花妍 Huayan	A212-218B283C132-137D115E285
W33	红珍珠 Wilgen’s Ruby	A214B275-283C139D115E285	ZY-11	墨碟心 Modiexin	A224-233B297-301C139D115E285
W35	锦缎 Germania	A207-214B283C132-137D115E285	ZY-12	粉精灵 Fenjingling	A214-235B278-283C135-137D115E285
R1	诺娃 Nova Zembla	A212-218B275-287C135-137D113-115E285	ZY-13	云之蜜语 Yunzhimiyu	A207-218B289C128-137D115E282-285
R2	粉黛 XXL	A222-264B301-313C132-137D115E285	ZY-14	阳春雪 Yangchunxue		A207-212B283C132-137D115E285
J1	普罗米修斯 Prominence	A224-239B287C135-137D113E282-285	ZY-15	宝钗 Baochai		A212-216B313C132-137D115E285
J2	紫色彩虹 Purple Rainbow	A224-239B287C135-137D113-115E282- 285	ZY-16	湘云 Xiangyun		A205-212B275C132-135D115E282-285
J3	紫色宫殿 Purple Dome	A228-243B287C137-141D113-115E282- 285	ZY-17	怡红 Yihong		A205-212B275C132-135D115E285
J4	红蝶 Red Monarch	A224B275C135-137D113E282-285	ZY-18	落雁 Luoyan		A227-239B297C137D115E282-285
J5	薰衣草王子 Lavendor Prince	A222-239B287C137D115E282-285	ZY-19	昆上初红 Kunshang Chuhong		A220-253B275C137-144D115E282-285
J6	草莓圣代 Strawberry Sundae	A224-227B287C137D113E282	ZY-20	罗裳 Luochang		A224-228B331-347C137D112-115E285
J7	珍珠姬 Sin Juhime	A231B286-289C137-141D115E282-285	ZY-21	翠湖紫鸥 Cuihu Ziou		A214-227B275C135-137D112-115E285
J8	玛丽亚 Marian	A239-243B287-301C137D113E285	ZY-22	翠湖彤云 Cuihu Tongyun		A222-227B275C135-137D112-115E285
J9	甜心 Sweet heart	A222-224B287C132-137D113-115E282- 285	ZY-23	翠湖涟漪 Cuihu Lianyi		A212-220B297C137-141D115E282-285
J10	阿特拉斯 Atlas	A224B307-313C135-137D113E285

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