Double Populations Genetic Mapping of the Main QTL Locus qDCA.C8-1 for the Curd Appearance Trait of Cauliflower

doi:10.16420/j.issn.0513-353x.2024-0944

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (9): 2343-2352.doi: 10.16420/j.issn.0513-353x.2024-0944

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Double Populations Genetic Mapping of the Main QTL Locus qDCA.C8-1 for the Curd Appearance Trait of Cauliflower

SHENG Xiaoguang¹, YU Huifang¹, WANG Jiansheng¹, SHEN Yusen¹, SONG Mengfei¹, QIAO Shuting², LIN Fan³, DU Sifan⁴, LI Jiaojiao³, GU Honghui¹^,^*()

¹ Institute of Vegetables， Zhejiang Academy of Agricultural Sciences， Hangzhou 310021， China
² Sanya Institute， China Agricultural University，Sanya， Hainan 572025， China
³ College of Horticultural Science， Zhejiang Agriculture and Forestry University， Hangzhou 311300， China
⁴ College of Life Sciences， China Jiliang University， Hangzhou 310018， China

Received:2025-03-20 Revised:2025-05-26 Online:2025-09-25 Published:2025-09-24
Contact: GU Honghui

Abstract

Abstract:

This study focused on Brassica oleracea crops，particularly cauliflower，and performed genetic mapping analysis of the curd appearance trait. By constructing two F₂ genetic segregating populations and utilizing a QTL mapping approach based on high-density genetic maps，a major locus（qDCA.C8-1）controlling cauliflower curd appearance was successfully mapped on chromosome 8. Subsequently，an F_2:3 sub-segregating population targeting the qDCA.C8-1 foreground locus was developed. Using bulked segregant analysis（BSA）sequencing technology，the target locus was re-mapped，further confirming its linkage with the curd appearance trait. The qDCA.C8-1 locus demonstrated consistent and significant genetic effects across different genetic populations，and its reliability was verified through KASP markers.

Key words: cauliflower, curd appearance, two genetic populations, gene mapping

SHENG Xiaoguang, YU Huifang, WANG Jiansheng, SHEN Yusen, SONG Mengfei, QIAO Shuting, LIN Fan, DU Sifan, LI Jiaojiao, GU Honghui. Double Populations Genetic Mapping of the Main QTL Locus qDCA.C8-1 for the Curd Appearance Trait of Cauliflower[J]. Acta Horticulturae Sinica, 2025, 52(9): 2343-2352.

Figures/Tables 5

Fig. 1 Phenotypic distribution of curd appearance trait in parents of C4101，J1401，R06 and their constructed double F2 populations A：Curd appearance phenotype of female parent（cauliflower C4101），male parents of Chinese kale（J1401）and broccoli landrace（R06）；B，C：CJF2 population and its parents；D，E：CRF2 population and its parents

Table 1 QTLs associated with curd appearance trait of cauliflower

群体 Populations	染色体 Chromosomes	QTL位点 QTL locus	位置/cM Position	阈值 LOD	加性效应 Additive effect	贡献率/% R²
CJF₂	1	1	1 198.01	11.09	-4.71	15.21
	2	2	1 018.61	4.61	2.39	4.40
	7	3	790.91	3.07	1.81	2.50
	8	4	522.61	9.42	3.52	8.95
CRF₂	1	1	609.11	2.79	-2.88	3.05
	3	2	696.31	2.85	-2.87	3.11
	4	3	7.31	5.04	3.24	4.70
	4	4	219.61	3.59	2.93	3.38
	5	5	237.81	6.17	-3.59	7.10
	6	6	760.31	3.93	2.90	3.69
	7	7	562.81	3.05	4.31	4.03
	8	8	567.11	3.08	-2.36	3.01
	8	9	878.11	5.88	3.63	6.43
	9	10	898.01	3.15	-2.76	2.98

Fig. 2 BSA analysis of extreme individuals with curd appearance trait in cauliflower F2:3 population A：Parent genome SNP for BSA analysis；B：Δ（SNP-index）plots of F2:3 BSA-seq results

Table 2 Primer sequence information for KASP markers FC84287 and RC84556

引物名称 Primers	变异位点 Variation locus	序列信息（5′-3′） Sequencing information
FC84287	T/A	F_HEX：GAAGGTGACCAAGTTCATGCTTATGGAAATCTGCTAGTTT
		F_FAM：GAAGGTCGGAGTCAACGGATTTATGGAAATCTGCTAGTTA
		R：TCGTATATATCCACTCCGAACT
RC84556	C/A	F_HEX：GAAGGTGACCAAGTTCATGCTCAGCCACGGATGTAAAGTCTTCC
		F_FAM：GAAGGTCGGAGTCAACGGATTCAGCCACGGATGTAAAGTCTTCA
		R：TAGAACACGCTACTTGGTTGCAGG

Fig. 3 KASP genotyping of F2:3 population using markers FC84287 and RC84556 A，B：FC84287 and RC84556 markers genotyping result；C：Distribution of curd appearance trait among three genotypes（a：C4101 genotype；b：J1401genotype；h：Heterozygous genotype）. NTC：H2O，black control. Different lowercase letters indicate significant differences at 0.05 level

References 30

[1]	Azpeitia E, Tichtinsky G, Le Masson M, Serrano-Mislata A, Lucas J, Gregis V, Gimenez C, Prunet N, Farcot E, Kater M M, Bradley D, Madueño F, Godin C, Parcy F. 2021. Cauliflower fractal forms arise from perturbations of floral gene networks. Science, 373 (6551):192-197. doi: 10.1126/science.abg5999 pmid: 34244409
[2]	Belser C, Istace B, Denis E, Dubarry M, Baurens F C, Falentin C, Genete M, Berrabah W, Chèvre A M. 2018. Chromosome-scale assemblies of plant genomes using nanopore long reads and optical maps. Nature Plants,4:879-887.
[3]	Blümel M, Dally N, Jung C. 2015. Flowering time regulation in crops-what did we learn from Arabidopsis? Current Opinion in Biotechnology,32:121-129.
[4]	Cao W, Cao B, Wang X, Bai J, Xu Y, Zhao J, Li X, He Y, Hu S. 2020. Alternatively spliced BobCAL transcripts alter curd morphotypes in a collection of Chinese cauliflower accessions. Horticulture Research, 7 (1):160.
[5]	Chen R, Chen K, Yao X, Zhang X, Yang Y, Su X, Lyu M, Wang Q, Zhang G, Wang M, Li Y, Duan L, Xie T, Li H, Yang Y, Zhang H, Guo Y, Jia G, Ge X, Sarris P, Sun D. 2024. Genomic analyses reveal the stepwise domestication and genetic mechanism of curd biogenesis in cauliflower. Nature Genetics, 56 (6):1235-1244. doi: 10.1038/s41588-024-01744-4 pmid: 38714866
[6]	Chiu L, Zhou X, Burke S, Wu X, Prior R, Li L. 2010. The purple cauliflower arises from activation of a MYB transcription factor. Plant Physiology, 154 (3):1470-1480. doi: 10.1104/pp.110.164160 pmid: 20855520
[7]	Gao Jing, Qin Mengfan, Li Kun, Li Wu. 2024. Visualization analysis of research progress on genetic characteristics of yield traits in maize. Chinese Agricultural Science Bulletin, 40 (16):156-164. (in Chinese) doi: 10.11924/j.issn.1000-6850.casb2024-0032
	高婧, 秦梦凡, 李坤, 李武. 2024. 玉米产量性状遗传特性研究进展的可视化分析. 中国农学通报, 40 (16):156-164. doi: 10.11924/j.issn.1000-6850.casb2024-0032
[8]	Guo N, Wang S, Gao L, Liu Y, Wang X, Lai E, Duan M, Wang G, Li J, Yang M, Zong M, Han S, Pei Y, Borm T, Sun H, Miao L, Liu D, Yu F, Zhang W, Ji H, Zhu C, Xu Y, Bonnema G, Li J, Fei Z, Liu F. 2021. Genome sequencing sheds light on the contribution of structural variants to Brassica oleracea diversification. BMC Biology, 19 (1):93.
[9]	He Q, Zhi H, Tang S, Xing L, Wang S, Wang H, Zhang A, Li Y, Gao M, Zhang H, Chen G, Dai S, Li J, Yang J, Liu H, Zhang W, Jia Y, Li S, Liu J, Qiao Z, Guo E, Jia G, Liu J, Diao X. 2021. QTL mapping for foxtail millet plant height in multi-environment using an ultra-high density bin map. Theoretical and Applied Genetics,134:557-72.
[10]	Jia Junzhong, Tian Liping, Xue Lin, Wei Yinong. 2010. Dynamic QTL and correlated characters of tomato soluble solid content. Hereditas (Beijing), 32 (10):1077-1083. (in Chinese)
	贾俊忠, 田丽萍, 薛琳, 魏亦农. 2010. 番茄可溶性固形物的动态QTL及相关性状. 遗传, 32 (10):1077-1083.
[11]	Kempin S, Savidge B, Yanofsky M. 1995. Molecular basis of the cauliflower phenotype in Arabidopsis. Science, 267 (5197):522-525. doi: 10.1126/science.7824951 pmid: 7824951
[12]	Li N, Yang R, Shen S X, Zhao J J. 2024. Molecular mechanism of flowering time regulation in Brassica rapa:similarities and differences with Arabidopsis. Horticultural Plant Journal, 10 (3):615-628.
[13]	Li Xixiang, Fang Zhiyuan. 2008. Descriptors and data standard for cauliflower(Brassica oleracea L. var. botrytis L. and Brassica oleracea L. var. italica Plenck). Beijing: China Agricultural Press:58-60. (in Chinese).
	李锡香, 方智远. 2008. 花椰菜和青花菜种质资源描述规范和数据标准. 北京: 中国农业出版社:58-60.
[14]	Mackay T. 2009. Q & A:genetic analysis of quantitative traits. Journal of Biology,83:23.
[15]	Shan H Y, Cheng J, Zhang R, Yao X, Kong H Z. 2019. Developmental mechanisms involved in the diversification of flowers. Nature Plants,5:917-923.
[16]	Shen Y, Yang Y, Xu E, Ge X, Xiang Y, Li Z. 2018. Novel and major QTL for branch angle detected by using DH population from an exotic introgression in rapeseed(Brassica napus L.). Theoretical and Applied Genetics,131:67-78.
[17]	Sheng X, Cai S, Shen Y, Yu H, Wang J, Qiao S, Lin F, Gu H. 2024. QTL analysis and fine mapping of a major QTL and identification of candidate genes controlling curd setting height in cauliflower. Vegetable Research,4:e008
[18]	Smith L, King G. 2000. The distribution of BoCAL-a alleles in Brassica oleracea is consistent with a genetic model for curd development and domestication of the cauliflower. Molecular Breeding, 6 (6):603-613.
[19]	Sun Deling, Yao Xingwei, Chen Rui, Yang Yingxia, Shan Xiaozheng. 2025. Review and prospects of cauliflower breeding during the past 50 years in China. Acta Horticulturae Sinica, 52 (5):1159-1179. (in Chinese) doi: 10.16420/j.issn.0513-353x.2025-0177
	孙德岭, 姚星伟, 陈锐, 杨迎霞, 单晓政. 2025. 国花椰菜育种50 年回顾与进展. 园艺学报, 52 (5):1159-1179. doi: 10.16420/j.issn.0513-353x.2025-0177
[20]	Tan H, Wang X, Fei Z, Li H, Tadmor Y, Mazourek M, Li L. 2020. Genetic mapping of green curd gene Gr in cauliflower. Theoretical and Applied Genetics, 133 (1):353-364. doi: 10.1007/s00122-019-03466-2 pmid: 31676958
[21]	Wang X, Han B, Sun Y, Kang X, Zhang M, Han H, Zhou S, Liu W, Lu Y, Yang X, Li X, Zhang J, Liu X, Li L. 2022. Introgression of chromosome 1P from Agropyron cristatum reduces leaf size and plant height to improve the plant architecture of common wheat. Theoretical and Applied Genetics,135:1951-1963.
[22]	Wen Songqin, Li Jialin, Chi Zhuoheng, Xia Yan, Wang Shuming, Wu Di, Hao Yawen, Guo Qigao, Liang Guolu, Jing Danlong. 2024. The role of light and temperature related genes alternative splicing in regulating plant flowering time. Acta Horticulturae Sinica, 51 (8):1949-1963. (in Chinese) doi: 10.16420/j.issn.0513-353x.2023-0946
	文宋琴, 李佳霖, 池卓恒, 夏燕, 王淑明, 吴頔, 郝雅雯, 郭启高, 梁国鲁, 景丹龙. 2024. 光和温度对开花时间基因可变剪接的影响研究进展. 园艺学报, 51 (8):1949-1963.
[23]	Xin X Y, Li P R, Zhao X Y, Yu Y J, Wang W H, Jin G H, Wang J H, Sun L L, Zhang D H, Zhang F L, Yu S C, Su T B. 2024. Temperature- dependent jumonji demethylase modulates flowering time by targeting H3K36me2/ 3 in Brassica rapa. Nature Communication, 15 (1):5470.
[24]	Shi Y, Song B, Liang Q, Su D, Lu W, Liu Y, Li Z. 2023. Molecular regulatory events of flower and fruit abscission in horticultural plants. Horticultural Plant Journal, 9 (5):867-883.
[25]	Zhang C, Zhou Q, Liu W, Wu X, Li Z, Xu Y, Li Y, Imaizumi T, Hou X, Liu T. 2022. BrABF3 promotes flowering through the direct activation of CONSTANS transcription in Pak Choi. Plant Journal, 111 (1):134-148.
[26]	Zhao J, Becker H, Ding H, Zhang Y, Zhang D, Ecke W. 2005. QTL of three agronomically important traits and their interactions with environment in a European × Chinese rapeseed population. Acta Genetica Sinica, 32 (9):969-978.
[27]	Zhao Z, Sheng X, Yu H, Wang J, Shen Y, Gu H. 2020a. Identification of candidate genes involved in curd riceyness in cauliflower. International Journal of Molecular Sciences,21:1999.
[28]	Zhao Z, Sheng X, Yu H, Wang J, Shen Y, Gu H. 2020b. Identification of QTLs associated with curd architecture in cauliflower. BMC Plant Biology,20:117.
[29]	Zhou X, Sun T, Wang N, Ling H, Lu S, Li L. 2011. The cauliflower Orange gene enhances petiole elongation by suppressing expression of eukaryotic release factor 1. New Phytologist, 190 (1):89-100.
[30]	Zu Feng, Zhao Kaiqin, Zhang Yunyun, Tian Zhengshu, Liu Yajun, Xi Junyu, Su Zhengqi, Fu Minglian. 2019. QTL mapping of flowering time and maturity time in Brassica napus L. Journal of Southern Agriculture, 50 (3):500-505. (in Chinese)
	俎峰, 赵凯琴, 张云云, 田正书, 刘亚俊, 奚俊玉, 束正齐, 符明联. 2019. 甘蓝型油菜的花期与生育期QTL定位. 南方农业学报, 50 (3):500-505.

Double Populations Genetic Mapping of the Main QTL Locus qDCA.C8-1 for the Curd Appearance Trait of Cauliflower

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