Analysis of Genetic Mechanism of Grape Distant Hybridization Based on Population Transcriptome

doi:10.16420/j.issn.0513-353x.2024-0115

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (4): 835-845.doi: 10.16420/j.issn.0513-353x.2024-0115

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Analysis of Genetic Mechanism of Grape Distant Hybridization Based on Population Transcriptome

WANG Yi¹^,², LIANG Zhenchang¹^,²^,^*()

¹ Beijing Key Laboratory of Grape Science and Enology，State Key Laboratory of Plant Diversity and Specialty Crops，Institute of Botany，Chinese Academy of Sciences，Beijing 100093，China
² China National Botanical Garden，Beijing 100093，China

Received:2024-12-17 Revised:2025-02-09 Online:2025-05-08 Published:2025-04-25
Contact: LIANG Zhenchang

Abstract

Abstract:

The main grape varieties for wineamking are all belongs to Vitis vinifera，and the genetic background is relative narrow. This study investigated the transcriptome diversity of grape berries in 51 accessions，including wild species，Vitis. vinifera，V. labrusca × V. vinifera and interspecific hybrid at green hard，veraison and ripening stages. The result indicated that the diversity of V. vinifera was extremely low and self-incompatibility was severe. Wild accessions maintained greater diversity，while interspecific hybrids can effectively bring these diversities to the cultivated varieties. Gene expression cluster and phenotypic data analysis also revealed that distant hybridization of cultivated and wild species could obtain good berry quality and strong resistance. In addition，transcriptome based diversity analysis can provide more information，and can serve as a supplement to traditional methods. In summary，wild varieties play an important role in grape breeding，and distant hybridization is an important way to recover grape population diversity.

Key words: grape, breeding, gene expression, diversity, distant hybridization

WANG Yi, LIANG Zhenchang. Analysis of Genetic Mechanism of Grape Distant Hybridization Based on Population Transcriptome[J]. Acta Horticulturae Sinica, 2025, 52(4): 835-845.

Figures/Tables 8

Fig. 1 Coefficient of variation distribution of four grapes in green hard，veraison，and ripening developmental stages W：Wild species；H：Interspecific hybrids；V：Vitis. vinifera；L：Vitis. labrusca × Vitis. vinifera. The same below

Fig. 2 Genetics diversity and inbreeding coefficient analysis of four grape groups A：Genetics diversity（π）analysis based on SNP information in expression region of four groups. B：Heterozygous SNP rate of four groups. C：Inbreeding coefficient comparison of all the four groups. Different lowercase letters indicate significant differences between different groups（P < 0.05）

Fig. 3 The distribution of new annotated coding and non-coding RNAs on the genome GH：Green hard；VE：Veraison；R：Ripening. The same below

Fig. 4 The number of new-transcripts of the four grape groups in three berry development stages

Fig. 5 The shared and the similar rate of the new annotated genes based on different types of grape transcripts

Fig. 6 The shared and specific gene families of the four grape groups based on the transcripts assemble

Fig. 7 Gene expression（Z-score）cluster and correlation analysis among wild and cultivated grape accessions

Fig. 8 Sugar and acid content in four grape groups Different lowercase letters indicate significant differences of sugar or acid content among different groups（P < 0.05）

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Analysis of Genetic Mechanism of Grape Distant Hybridization Based on Population Transcriptome

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