Grapevine VvARF18 Regulates Fruit Expansion and Screening of its Interacting Proteins

doi:10.16420/j.issn.0513-353x.2024-0594

Acta Horticulturae Sinica ›› 2025, Vol. 52 ›› Issue (4): 821-834.doi: 10.16420/j.issn.0513-353x.2024-0594

• Genetic & Breeding · Germplasm Resources · Molecular Biology • Previous Articles Next Articles

Grapevine VvARF18 Regulates Fruit Expansion and Screening of its Interacting Proteins

LIANG Jing，ZENG Baozhen，LIANG Guoping，MAO Juan，and CHEN Baihong*（bhch@gsau.edu.cn）

College of Horticulture，Gansu Agricultural University，Lanzhou 730000

Received:2024-10-11 Revised:2025-03-10 Online:2025-04-25 Published:2025-04-25
Contact: CHEN Baihong

Abstract

Abstract: In order to investigate the molecular mechanism of grape VvARF18 in fruit development，‘Red Globe’grapes（Vitis vinifera‘Red Globe’）were used as materials，the VvARF18 gene was cloned，and analyzed for its bioinformatics，and the gene was heterologously overexpressed in tomato to verify its effect on fruit development. The CDS of the VvARF18 gene was 2 109 bp in length，coding for 703 amino acids，with a pI of 6.50. Phylogenetic analysis showed that VvARF18 was the closest related to the VrARF18 protein of Vitis riparis；the gene was heterologously overexpressed in tomato. Expression，the longitudinal and transverse diameters of T₃ generation transgenic tomato fruits were significantly higher than those of the wild type，and its volume and single fruit mass were significantly increased，suggesting that VvARF18 has a positive regulatory effect on fruit expansion；transcriptional activation assay found that VvARF18 was not toxic but had self-activating activity，and screening of proteins interacting with its conserved functional structural domain B3-Auxin revealed a potential interplay relationship with the signaling complex VvCOP9.

Key words: grape, VrARF18, subcelluar localization, functional verification, yeast two-hybrid

CLC Number:

S 663.1

LIANG Jing, ZENG Baozhen, LIANG Guoping, MAO Juan, CHEN Baihong. Grapevine VvARF18 Regulates Fruit Expansion and Screening of its Interacting Proteins[J]. Acta Horticulturae Sinica, 2025, 52(4): 821-834.

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Grapevine VvARF18 Regulates Fruit Expansion and Screening of its Interacting Proteins

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