Studies on the Physiological Indexes and Endogenous Hormones Variation During Flower Development in Michelia crassipes

doi:10.16420/j.issn.0513-353x.2023-0562

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (8): 1881-1890.doi: 10.16420/j.issn.0513-353x.2023-0562

• Cultivation·Physiology & Biochemistry • Previous Articles Next Articles

Studies on the Physiological Indexes and Endogenous Hormones Variation During Flower Development in Michelia crassipes

GUO Qunyan¹^,², LIU Caixian¹^,²^,^*(), YU Qiuxiu¹, ZHANG Hua¹, ZHENG Meiting¹

¹ College of Landscape Architecture，Central South University of Forestry and Technology，Changsha 410000，China
² Hunan Big Data Engineering Technology Research Center of Natural Protected Areas Landscape Resources，Changsha 410004，China

Received:2024-01-05 Revised:2024-05-23 Online:2024-08-25 Published:2024-08-22
Contact: LIU Caixian

Abstract

Abstract:

To study the physiological changes and endogenous hormones variation during the flower development of Michelia crassipes，the flower buds from green flower bud stage，purple flower bud stage and the first-open stage were collected，to measure the physiological indexes and the variant endogenous hormones. The results showed that the contents of soluble sugar，soluble protein，chlorophyll and POD activity were decreased during the development of flower bud. The contents of free proline，malondialdehyde，anthocyanin and SOD activity showed the highest value in purple bud stage. According to the UPLC-ESI-MS/MS analysis，a total of 49 hormone substances belonging to 8 classes were detected during the whole developmental process. The flower buds in green flower bud stage accumulated relatively more auxin，salicylic acid（SA）and abscisic acid（ABA），while in purple flower bud stage，the auxin content accounted for the largest proportion of total hormones. The auxin and jasmonic acid（JA） were the predominant hormones in the first-open stage. Additionally，the gibberellin（GA）and ethylene（ETH）contents were increased during the flower development，but the cytokinin（CK）and strigolactones（SLs）contents were decreased. The hormones with significant difference in the content were screened and significantly enriched in the biosynthesis of secondary metabolites pathway. These results indicated that the different physiological activities and hormone interaction patterns worked cooperatively to regulate the flower organs development and anthocyanin accumulation during flower developmental process. Especially，the soluble sugar，soluble protein，auxin，abscisic acid and jasmonic acid might play an important role.

Key words: Michelia crassipes, flower development, physiology, endogenous hormone

GUO Qunyan, LIU Caixian, YU Qiuxiu, ZHANG Hua, ZHENG Meiting. Studies on the Physiological Indexes and Endogenous Hormones Variation During Flower Development in Michelia crassipes[J]. Acta Horticulturae Sinica, 2024, 51(8): 1881-1890.

Figures/Tables 8

References 35

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花发育期 Flower developmental stage	花芽长/mm Flower bud length	花芽宽/mm Flower bud width	色素含量/（mg · g^-1）Pigment content
花发育期 Flower developmental stage	花芽长/mm Flower bud length	花芽宽/mm Flower bud width	类胡萝卜素 Carotenoid	叶绿素 Chlorophyll	花青素 Anthocyanin
绿蕾期 Green bud stage	5.04 ± 0.16 c	2.71 ± 0.11 c	0.03 ± 0.00 a	0.11 ± 0.01 a	0.12 ± 0.01 b
紫蕾期 Purple bud stage	13.92 ± 0.44 b	6.77 ± 0.24 b	0.04 ± 0.00 a	0.11 ± 0.01 a	8.24 ± 0.22 a
初开期 The first-open stage	24.57 ± 0.24 a	13.54 ± 0.25 a	0.01 ± 0.00 b	0.02 ± 0.00 b	7.68 ± 0.18 a

花发育期 Flower developmental stage	花芽长/mm Flower bud length	花芽宽/mm Flower bud width	色素含量/（mg · g^-1）Pigment content
花发育期 Flower developmental stage	花芽长/mm Flower bud length	花芽宽/mm Flower bud width	类胡萝卜素 Carotenoid	叶绿素 Chlorophyll	花青素 Anthocyanin
绿蕾期 Green bud stage	5.04 ± 0.16 c	2.71 ± 0.11 c	0.03 ± 0.00 a	0.11 ± 0.01 a	0.12 ± 0.01 b
紫蕾期 Purple bud stage	13.92 ± 0.44 b	6.77 ± 0.24 b	0.04 ± 0.00 a	0.11 ± 0.01 a	8.24 ± 0.22 a
初开期 The first-open stage	24.57 ± 0.24 a	13.54 ± 0.25 a	0.01 ± 0.00 b	0.02 ± 0.00 b	7.68 ± 0.18 a

花发育期 Flower developmental stage	可溶性糖/ （mg · g^-1） Soluble sugar	可溶性蛋白/ （mg · g^-1） Soluble protein	MDA/ （μmol · g^-1）	游离脯氨酸/（μg · g^-1） Free proline	SOD/ （U · min^-1 · g^-1 FW）	POD/ （U · min^-1 · g^-1 FW）
绿蕾期 Green bud stage	0.08 ± 0.00 a	2.90 ± 0.02 a	12.59 ± 2.40 c	205.55 ± 3.55 c	461.67 ± 2.89 b	958.72 ± 33.92 a
紫蕾期 Purple bud stage	0.07 ± 0.00 a	2.36 ± 0.01 b	202.05 ± 0.71 a	453.22 ± 23.82 a	667.98 ± 72.31 a	688.57 ± 22.86 b
初开期 The first-open stage	0.06 ± 0.00 b	1.74 ± 0.09 c	114.76 ± 0.06 b	264.66 ± 12.53 b	77.64 ± 8.97 c	214.00 ± 7.21 c

花发育期 Flower developmental stage	可溶性糖/ （mg · g^-1） Soluble sugar	可溶性蛋白/ （mg · g^-1） Soluble protein	MDA/ （μmol · g^-1）	游离脯氨酸/（μg · g^-1） Free proline	SOD/ （U · min^-1 · g^-1 FW）	POD/ （U · min^-1 · g^-1 FW）
绿蕾期 Green bud stage	0.08 ± 0.00 a	2.90 ± 0.02 a	12.59 ± 2.40 c	205.55 ± 3.55 c	461.67 ± 2.89 b	958.72 ± 33.92 a
紫蕾期 Purple bud stage	0.07 ± 0.00 a	2.36 ± 0.01 b	202.05 ± 0.71 a	453.22 ± 23.82 a	667.98 ± 72.31 a	688.57 ± 22.86 b
初开期 The first-open stage	0.06 ± 0.00 b	1.74 ± 0.09 c	114.76 ± 0.06 b	264.66 ± 12.53 b	77.64 ± 8.97 c	214.00 ± 7.21 c

花发育期 Flower developmental stage	脱落酸 ABA	生长素 IAA	细胞分裂素 CK	赤霉素 GA	茉莉酸 JA
绿蕾期 Green bud stage	1 320.68 ± 51.73 a	953.17 ± 25.27 c	71.59 ± 3.19 a	2.61 ± 0.19 b	49.29 ± 4.15 b
紫蕾期 Purple bud stage	257.38 ± 18.01 c	11 089.49 ± 292.45 a	11.75 ± 0.97 b	3.10 ± 0.15 b	38.77 ± 3.64 b
初开期 The first-open stage	994.26 ± 56.40 b	6 528.16 ± 114.63 b	10.25 ± 0.59 b	4.13 ± 3.17 a	6 000.12 ± 46.16 a
花发育期 Flower developmental stage	水杨酸 SA	独脚金内酯 SL	乙烯类 ETH	总含量 Total content
绿蕾期 Green bud stage	1 072.38 ± 28.64 a	2.50 ± 0.29 a	24.25 ± 1.41 c	3 469.72 ± 63.61 c
紫蕾期 Purple bud stage	176.79 ± 0.83 b	1.28 ± 0.21 b	68.33 ± 1.74 b	11 577.28 ± 284.23 b
初开期 The first-open stage	141.56 ± 8.09 b	1.23 ± 0.14 b	139.50 ± 2.20 a	13 678.49 ± 36.20 a

Studies on the Physiological Indexes and Endogenous Hormones Variation During Flower Development in Michelia crassipes

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激素种类 Hormone	名称 Name	st1 vs. st2	st1 vs. st3	st2 vs. st3
茉莉酸 JA	氧化戊烯基环戊烷丁酸 3-oxo-2-(2-(Z)-Pentenyl)cyclopentane-1-butyric acid	上调 Up	—	下调 Down
	12-氧-植物-二烯酸 cis(+)-12-Oxophytodienoic acid	下调 Down	下调 Down	上调 Up
	二氢茉莉酸 Dihydrojasmonic acid	下调 Down	—	—
	茉莉酸甲酯 Methyl jasmonate	—	上调 Up	上调 Up
	茉莉酸-苯丙氨酸 N-[(-)-Jasmonoyl]-(l)-phenalanine	—	上调 Up	上调 Up
生长素 IAA	吲哚-3-乙酸甲酯 Methyl indole-3-acetate	下调 Down	下调 Down	—
	吲哚-3-丁酸 Indole-3-butyric acid	上调 Up	—	下调 Down
	3-吲哚乙酰胺 3-Indole acetamide	上调 Up	上调 Up	下调 Down
	吲哚-3-乳酸 Indole-3-lactic acid	下调 Down	下调 Down	下调 Down
	色胺 Tryptamine	上调 Up	上调 Up	—
	吲哚乙酸-天冬氨酸 Indole-3-acetyl-L-aspartic acid	下调 Down	下调 Down	—
	吲哚-3-甲酸 Indole-3-carboxylic acid	下调 Down	下调 Down	—
	吲哚乙酸-亮氨酸 N-(3-Indolylacetyl)-L-leucine	下调 Down	下调 Down	—
	N-（3-吲哚乙酰基）-L-丙氨酸 N-(3-Indolylacetyl)-L-alanine	下调 Down	下调 Down	—
	吲哚-3-甲醛 Indole-3-carboxaldehyde	—	上调 Up	—
	吲哚-3-乙酸 Indole-3-acetic acid	—	上调 Up	上调 Up
	吲哚乙酸-甘氨酸 Indole-3-acetyl glycine	—	下调 Down	—
细胞分裂素 CK	玉米素核苷 trans-Zeatin riboside	上调 Up	上调 Up	—
	反式玉米素-9-Β-葡萄糖苷 trans-Zeatin-O-glucoside	下调 Down	下调 Down	—
	双氢玉米素-7-糖苷 Dihydrozeatin-7-glucoside	上调 Up	上调 Up	上调 Up
	N6-异戊烯腺嘌呤 N6-isopentenyladenine	下调 Down	下调 Down	—
	顺式玉米素-9-葡糖苷 cis-Zeatin-9-glucoside	下调 Down	下调 Down	上调 Up
	6-糠氨基嘌呤 Kinetin	上调 Up	—	下调 Down
	4-[（9H-嘌呤-6-基氨基）甲基]苯酚 para-Topolin	下调 Down	下调 Down	—
	2-甲硫基异戊烯基腺嘌呤核苷 2-Methylthio-N6-isopentenyladenosine	下调 Down	下调 Down	—
	二氢玉米素 Dihydrozeatin	—	上调 Up	上调 Up
	双氢玉米核苷-O-糖苷 Dihydrozeatin-O-glucoside riboside	—	上调 Up	上调 Up
	反式玉米素 trans-Zeatin	—	上调 Up
	异戊烯腺嘌呤核苷 N6-isopentenyladenosine	—	下调 Down	下调 Down
	双氢玉米素核苷 Dihydrozeatin ribonucleoside	—	—	上调 Up
赤霉素 GA	赤霉素20 Gibberellin A20	下调 Down	—	—
	赤霉素3 Gibberellin A3	上调 Up	上调 Up	—
独角金内酯 SL	5-脱氧独脚金醇 5-Deoxystrigol	—	下调 Down	—
水杨酸 SA	水杨酸-2-O-β-葡萄糖苷 Salicylic acid 2-O-β-glucoside	下调 Down	—	—
	水杨酸 Salicylic acid	下调 Down	下调 Down	上调 Up
脱落酸 ABA	脱落酸 Abscisic acid	下调 Down	—	上调 Up
	脱落酸葡萄糖酯 ABA-glucosyl ester	上调 Up	上调 Up	—

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