Functional Study of MrSPL4 Gene in Response to Drought and Low Temperature Stress in Chinese Bayberry

doi:10.16420/j.issn.0513-353x.2023-0258

Acta Horticulturae Sinica ›› 2024, Vol. 51 ›› Issue (5): 927-938.doi: 10.16420/j.issn.0513-353x.2023-0258

Functional Study of MrSPL4 Gene in Response to Drought and Low Temperature Stress in Chinese Bayberry

WU Xiangqi¹^,³, SUN Li¹, YU Zheping¹, YU Qinpei¹^,³, LIANG Senmiao¹, ZHENG Xiliang¹, QI Xingjiang¹^,², ZHANG Shuwen¹^,^*()

¹ Institute of Horticulture，Zhejiang Academy of Agricultural Sciences，Hangzhou 310021，China
² Xianghu Laboratory，Hangzhou 311231，China
³ College of Chemistry and Life Sciences，Zhejiang Normal University，Jinhua，Zhejiang 321004，China

Received:2023-11-30 Revised:2024-02-29 Online:2024-05-25 Published:2024-05-29
Contact: *（E-mail：hizhangshuwen@163.com）

Abstract

Abstract:

To investigate the function of MrSPL4 in response to drought and low temperature stress in Chinese bayberry，the cis-acting element of MrSPL4 promoter and treating MrSPL4-OE transgenic tobacco T₂ generation strain with drought and low temperature stress were analyzed，observing its seed germination rate and plant phenotype，and measuring plant physiological indexes to clarify the function of MrSPL4. The results showed that the promoter region of MrSPL4 contains cis-acting elements for drought induction，low temperature response，defense and stress response，and the other important cis-elements. Compared with wild-type tobacco（WT），under drought stress，MrSPL4-OE transgenic tobacco seed germination rate was significantly reduced. There was no significant difference in the reduction of plant water loss and wilting between WT and MrSPL4-OE. Except for a significant decrease in H₂O₂ content，there was no significant difference in other indicators. Transfer to normal conditions after low temperature stress，the germination rate of seeds significantly increased. Plant greening and wilting were reduced. SOD and POD enzyme activities increased significantly，while the content of MDA，H₂O₂，proline，and soluble sugar decreased significantly. The results indicated that MrSPL4 positively regulates the response process of tobacco to low temperature stress，improves its ability to tolerate low temperature，but inhibits the germination rate of tobacco seeds under drought stress.

Key words: Chinese bayberry, SPL gene, seed germination, drought stress, low-temperature stress

WU Xiangqi, SUN Li, YU Zheping, YU Qinpei, LIANG Senmiao, ZHENG Xiliang, QI Xingjiang, ZHANG Shuwen. Functional Study of MrSPL4 Gene in Response to Drought and Low Temperature Stress in Chinese Bayberry[J]. Acta Horticulturae Sinica, 2024, 51(5): 927-938.

Figures/Tables 7

Fig. 1 Relative expression levels of MrSPL4 in transgenic tobacco lines（MrSPL4-OE）and wild-type（WT） t-test，*** P < 0.001.

Table 1 Predicted cis-elements in the promoter of the MrSPL4 gene

顺式作用元件	数量	起始位置/bp	终止位置/bp
cis-Element	Number	Starting position	Ending position
赤霉素应答元件 Gibberellin responsiveness	1	193	203
干旱诱导元件 Drought inducibility	3	79	89
		1 450	1 460
		1 491	1 501
光反应元件 Light responsiveness	3	737	747
		1 434	1 444
		1 417	1 427
生长素响应元件 Auxin responsiveness	1	1 373	1 383
低温响应元件 Low-temperature responsiveness	2	251	261
		1 405	1 415
厌氧诱导元件 Anaerobic induction	2	275	285
		1 454	1 464
防御和胁迫响应元件 Defense and stress responsiveness	2	852	862
		1 428	1 438
茉莉酸甲酯响应元件 MeJA responsiveness	4	736	746
		1 462	1 472
		736	746
		1 462	1 472

Fig. 2 Germination of transgenic tobacco lines（MrSPL4-OE）and wild-type（WT）seeds under different concentrations of mannitol treatment（14 days） ** P < 0.01.

Fig. 3 Leaf physiological indicators of transgenic tobacco lines（MrSPL4-OE）and wild-type（WT）plants under drought stress ** P < 0.01.

Fig. 4 Germination rate of transgenic tobacco lines（MrSPL4-OE）and wild-type（WT）seeds under low-temperature treatment ** P < 0.01.

Fig. 5 Phenotypes of transgenic tobacco lines（MrSPL4-OE）and wild-type（WT）before and after low-temperature treatment

Fig. 6 Leaf physiological indicators of transgenic tobacco lines（MrSPL4-OE）and wild-type（WT）under low temperature stress * P < 0.05，** 0.01 < P < 0.05，*** P < 0.001.

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Functional Study of MrSPL4 Gene in Response to Drought and Low Temperature Stress in Chinese Bayberry

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