Preliminary Study on the Mechanism of Improvement of Resistance to Chinese Bayberry Twig Blight Disease by Brassinolide and Oligosaccharin

doi:10.16420/j.issn.0513-353x.2022-0873

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (10): 2229-2241.doi: 10.16420/j.issn.0513-353x.2022-0873

• Plant Protection • Previous Articles Next Articles

Preliminary Study on the Mechanism of Improvement of Resistance to Chinese Bayberry Twig Blight Disease by Brassinolide and Oligosaccharin

YU Zheping¹, YAN Xiujuan¹^,², ZHANG Shuwen¹, ZHENG Xiliang¹, SUN Li¹, REN Haiying¹, LIANG Senmiao¹, QI Xingjiang¹^,³^,^*()

¹ Institute of Horticulture，Zhejiang Academy of Agricultural Sciences，Hangzhou 310021，China
² College of Life Science，China Jiliang University，Hangzhou 310018，China
³ Xianghu Lab.，Hangzhou 311231，China

Received:2023-04-11 Revised:2023-08-07 Online:2023-10-25 Published:2023-10-31

Abstract

Abstract:

To explore the mechanism of induced resistance by immune inducers to Chinese bayberry （Myrica rubra）twig blight disease，young plants of‘Dongkui’were used as test materials to study the effects of exogenous inducers on the induction effect，growth index，chlorophyll content，antioxidant enzyme activity and expressions of pathogenesis-related genes in M. rubra after inoculation with twig blight strains. The results showed that 2,4-epibrassinolide（EBR）and oligosaccharins · plant activator protein（OPAP）could significantly reduce the twig blight disease index，with better effects of resistance induction（reaching 47.94% and 39.01%，respectively）. Both EBR and OPAP effectively promoted the growth of M. rubra，including plant height，leaf length and leaf area，which were higher than those of the control. Furthermore，EBR and OPAP significantly improved the contents of chlorophyll a，chlorophyll b and carotenoids on 28-day after inoculation，which were increased by 13.9%-26.8% compared with the control. Under the treatment of EBR and OPAP，the activities of SOD，POD and CAT significantly increased in a short time，and their activities were increased by 87.4%，106.5% and 77.9% compared with the control on 28-day after inoculation，while MDA content was lower than that of control. Both EBR and OPAP could significantly induce up-regulated expressions of M. rubra pathogenesis-related genes during 7-day to 28-day after inoculation，including MrPR1，MrPR2 and MrPR10，and their expressions were maintained at a high level. In conclusion，EBR and OPAP have better effects on the induced resistance to M. rubra twig blight disease，and their potential induction mechanisms may be closely related to the enhancement of antioxidant enzyme activity and the up-regulation of pathogenesis-related genes.

Key words: Chinese bayberry, inducer, brassinolide, oligosaccharin, twig blight disease, antioxidant enzyme activity, pathogenesis-related protein

YU Zheping, YAN Xiujuan, ZHANG Shuwen, ZHENG Xiliang, SUN Li, REN Haiying, LIANG Senmiao, QI Xingjiang. Preliminary Study on the Mechanism of Improvement of Resistance to Chinese Bayberry Twig Blight Disease by Brassinolide and Oligosaccharin[J]. Acta Horticulturae Sinica, 2023, 50(10): 2229-2241.

Figures/Tables 7

Table 1 Primer sequences for qRT-PCR

基因 Gene	上游引物（5′-3′） Forward primer	下游引物（5′-3′） Reverse primer
MrActin	AATGGAACTGGAATGGTCAAGGC	TGCCAGATCTTCTCCATGTCATCCCA
MrPR1	GCCTTGGTCCTTGATTCCGA	GGTCGAGCTCATCAACCCTT
MrPR2	GCTAACTGCCCTTCCGAGTT	AGCGTAACTATACGCGGTCG
MrPR10	GAACCGGCTGCAACTTTGAC	GACGACTAGTGGCACGTTGA

Table 2 Effects of 2,4-epibrassionolide（EBR）and oligosaccharins · plant activator protein（OPAP）treatments on twig blight disease in Chinese bayberry

诱导剂 Inducer	病情指数 Disease index	抗性诱导效果/% Resistance induction effect
对照 Control	54.42 ± 2.69 a	0
OPAP	33.19 ± 1.72 b	39.01 ± 2.27 b
EBR	28.33 ± 1.64 c	47.94 ± 2.56 a

Table 3 Effects of 2,4-epibrassionolide（EBR）and oligosaccharins·plant activator protein（OPAP）treatment on plant height of Chinese bayberry cm

诱导剂 Inducer	接种凋萎病菌后天数/d Days after inoculation with pathogen of twig blight disease
诱导剂 Inducer	0	7	14	21	28
对照 Control	45.3 ± 2.8 a	47.7 ± 2.9 a	50.2 ± 3.7 b	55.8 ± 1.6 b	57.1 ± 3.6 b
OPAP	44.7 ± 2.2 ab	49.3 ± 3.7 a	52.5 ± 2.2 b	61.6 ± 1.7 ab	67.8 ± 2.1 ab
EBR	42.4 ± 1.7 b	49.8 ± 1.1 a	58.5 ± 0.9 a	64.7 ± 2.6 a	69.4 ± 2.8 a

Fig. 1 Effects of 2,4-epibrassionolide（EBR）and oligosaccharins · plant activator protein（OPAP）treatments on leaf growth of Chinese bayberry Different lowercase letters indicate significant differences among different treatments at same treatment time. The same below.

Fig. 2 Effects of 2,4-epibrassionolide（EBR）and oligosaccharins · plant activator protein（OPAP）treatments on chlorophyll content in Chinese bayberry leaves

Fig. 3 Effects of 2,4-epibrassionolide（EBR）and oligosaccharins · plant activator protein（OPAP）treatments on activity of SOD，POD，CAT and MDA content in Chinese bayberry leaves

Fig. 4 Effects of 2,4-epibrassionolide（EBR）and oligosaccharins · plant activator protein（OPAP）treatments on the expression of MrPR1，MrPR2 and MrPR10 in Chinese bayberry

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Preliminary Study on the Mechanism of Improvement of Resistance to Chinese Bayberry Twig Blight Disease by Brassinolide and Oligosaccharin

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