Jasmonic Acid Carboxy Methyltransferase Gene RhJMT Regulates Petal Senescence in Rosa hybrida

doi:10.16420/j.issn.0513-353x.2022-0177

Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (5): 1025-1036.doi: 10.16420/j.issn.0513-353x.2022-0177

• Genetic & Breeding·Germplasm Resources·Molecular Biology • Previous Articles Next Articles

Jasmonic Acid Carboxy Methyltransferase Gene RhJMT Regulates Petal Senescence in Rosa hybrida

LIU Jiaqi¹, GONG Feifei¹, ZHANG Hao², JING Weikun¹^,², QU Suping², MA Nan¹, GAO Junping¹, SUN Xiaoming¹^,^*()

¹Beijing Key Laboratory of Development and Quality Control of Ornamental Crops，Department of Ornamental Horticulture，College of Horticulture，China Agricultural University，Beijing 100193，China
²National Engineering Research Center of Ornamental Horticulture，Flower Research Institute，Yunnan Academy of Agricultural Sciences，Kunming 650205，China

Received:2023-02-17 Revised:2023-04-27 Online:2023-05-25 Published:2023-05-31
Contact: SUN Xiaoming E-mail:xmsun@cau.edu.cn

Abstract

Abstract:

In order to understand the mechanism of MeJA in petal senescence，the rose variety ‘Samantha’was used as the test material，and the jasmonic acid carboxyl methyltransferase（JMT）gene RhJMT，a key enzyme in MeJA biosynthesis，was cloned in the petals. Homologous protein sequence alignment and phylogenetic tree analysis showed that RhJMT has a conserved Methylyransf_7 superfamily domain and belongs to the SABATH protein family. The results of real-time quantitative PCR showed that the expression of RhJMT increased significantly after petal senescence. The function of RhJMT was verified by virus-induced gene silencing technology. The results showed that silencing RhJMT significantly delayed petal senescence，and that exogenous application of MeJA could counteract the delaying effect of silencing the RhJMT gene. These results indicate that RhJMT can promote petal senescence and may act by controlling the concentration of MeJA.

Key words: Rosa hybrida, petal senescence, methyl jasmonate（MeJA）, jasmonate methyltransferase （JMT）, VIGS

CLC Number:

S685.12

LIU Jiaqi, GONG Feifei, ZHANG Hao, JING Weikun, QU Suping, MA Nan, GAO Junping, SUN Xiaoming. Jasmonic Acid Carboxy Methyltransferase Gene RhJMT Regulates Petal Senescence in Rosa hybrida[J]. Acta Horticulturae Sinica, 2023, 50(5): 1025-1036.

Figures/Tables 8

Table 1 Primers used in this study

用途 Function	引物名称 Primer name	引物序列（5′-3′） Sequence
克隆基因 Gene clone	RhJMT-F	GAGATAATGGAGGTAGAGCAAGTTCTTC
克隆基因 Gene clone	RhJMT-R	GCTTCATATTGTATACTTGCACG
病毒诱导的基因沉默 Virus induced gene silencing	TRV1-F	TTACAGGTTATTTGGGCTAG
	TRV1-R	CCGGGTTCAATTCCTTATC
	TRV2-F	TGGGAGATGATACGCTGTT
	TRV2-R	CCTAAAACTTCAGACACG
	TRV2-RhJMT-F	AAGGTTACCGAATTCTCTAGAGGTACTGTCCACTGACATAAGAGC
	TRV2-RhJMT-R	CTCGAGACGCGTGAGCTCGGTACCGCTTCATATTGTATACTTGCACG
实时荧光定量PCR	RhUBI2-F	GCCCTGGTGCGTTCCCAACTG
qRT-PCR	RhUBI2-R	CCTGCGTGTCTGTCCGCATTG
	qRT-RhJMT-F	GTACTGTCCACTGACATAAGAGC
	qRT-RhJMT-R	GCTTCATATTGTATACTTGCACG
	qRT-RhSAG12-F	AGCGGAGAAGCCTTTCAGTC
	qRT-RhSAG12-R	CAGCATGGTTCAGGCTGGTA
	qRT-RhSAG113-F	TGTGTCCTCTGTAACCAACAAGT
	qRT-RhSAG113-R	CTCCAACTAGACCGCAGCAA

Fig. 1 Alignment and analysis of JMT protein sequences from various plant species The box is selected for Methylyransf_7 superfamily’s conservative domain. Rh：Rosa hybrida；In：Ipomoea nil；Na：Nicotiana attenuata；Fv：Fragaria vesca；Gm：Glycine max；St：Solanum tuberosum.

Fig. 2 Phylogenetic tree of RhJMT and JMTs from other plant species

Fig. 3 The relative expression level of RhJMT in different tissues（A）and in different opening stages（B）of rose flowers

Fig. 4 Effect of silencing RhJMT on flower senescence in Rosa hybrida‘Samantha’ *** P < 0.001.

Fig. 5 Expression of RhSAG12 and RhSAG113 in RhJMT-silenced plants ** P < 0.01，*** P < 0.001.

Fig. 6 Effects of silencing RhJMT and exogenous MeJA treatment on petal senescence in Rosa hybrida‘Bride’

Fig. 7 Effects of silencing RhJMT and exogenous MeJA treatment on area of disc discoloration in Rosa hybrida‘Bride’ *** P < 0.001.

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Jasmonic Acid Carboxy Methyltransferase Gene RhJMT Regulates Petal Senescence in Rosa hybrida

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