VIGS Expression Vector Construction and Expression Analyses of SmMsrA Gene in Eggplant

doi:10.16420/j.issn.0513-353x.2015-0177

ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (8): 1495-1504.doi: 10.16420/j.issn.0513-353x.2015-0177

• Vegetables • Previous Articles Next Articles

VIGS Expression Vector Construction and Expression Analyses of SmMsrA Gene in Eggplant

ZHAO Zhen, LIU Fu-Zhong, ZHANG Ying, QI Dong-Xia, CHEN Yu-Hui, LIAN Yong

Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China

Online:2015-08-25 Published:2015-08-25

Abstract

Abstract: The coding sequence of methionine sulfoxide reductase A（SmMsrA）was amplified from eggplant（Solanum melongena L.）parthenocarpic line D-10 using reverse transcription polymerase chain reaction（RT-PCR）method. Through the Gateway recombination technology，three TRV（Tobacco rattle virus）-mediated VIGS expression vectors were constructed，pTRV2-SmMsrAi，containing different fragments of SmMsrA gene. The VIGS expression vectors were transformed into Agrobacterium strain GV3101，and then inoculated eggplant leaves by Agro-syringe. Phenotypes observation，TRV molecular detection and qRT-PCR thechnology were used to observe and evaluate the VIGS system for silencing the SmMsrA gene effection. The results showed that the positive control of silencing reporter gene PDS showed photo-bleaching phenotype in eggplant leaves；Silencing SmMsrA gene were observed pale yellow（mosaic）leaves and bore smaller fruits；The expression of SmMsrA gene was decreased obviously in the leaves and fruits of the infected eggplant plants. These results suggested that theSmMsrA gene was a related gene in positive regulation of fruit development in eggplant.

Key words: eggplant, SmMsrA gene, VIGS, Gateway technology, fruit development

CLC Number:

S 641.1

ZHAO Zhen, LIU Fu-Zhong, ZHANG Ying, QI Dong-Xia, CHEN Yu-Hui, LIAN Yong. VIGS Expression Vector Construction and Expression Analyses of SmMsrA Gene in Eggplant[J]. ACTA HORTICULTURAE SINICA, 2015, 42(8): 1495-1504.

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VIGS Expression Vector Construction and Expression Analyses of SmMsrA Gene in Eggplant

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