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Acta Horticulturae Sinica ›› 2023, Vol. 50 ›› Issue (2): 295-306.doi: 10.16420/j.issn.0513-353x.2021-1246

• Research Papers • Previous Articles     Next Articles

The Transcription Factor FvbHLH130 Activates Flowering in Fragaria vesca

SONG Yanhong1, CHEN Yaduo1, ZHANG Xiaoyu2, SONG Pan1, LIU Lifeng1, LI Gang1, ZHAO Xia1, ZHOU Houcheng1,*()   

  1. 1. Zhengzhou Fruit Research Institute,Chinese Academy of Agricultural Sciences,Zhengzhou 450009,China
    2. College of Horticulture,Henan Agricultural University,Xuchang,Henan 461101,China
  • Received:2022-08-23 Revised:2022-11-03 Online:2023-02-25 Published:2023-03-06
  • Contact: *(E-mail:zhouhoucheng@caas

Abstract:

The basic helix-loop-helix(bHLH)transcription factor family of strawberry was analyzed,and the transcription factor FvbHLH130 was identified in Fragaria vesca. qRT-PCR analysis in different tissues showed that FvbHLH130 was flower-specifically expressed. Overexpression of FvbHLH130 in Arabidopsis thaliana activated flowering in transgenic lines. The full-length coding sequence of FvbHLH130 was 960 bp,encoding a 319-amino-acid protein. The prediction of conserved domain of FvbHLH130 showed that 247-297 amino acids were bHLH binding domain. The promoter of FvbHLH130 contained hormone,stress- and low temperature-response elements,which indicated FvbHLH130 might be induced by biotic and abiotic stress. The amino acids sequence of FvbHLH130 revealed up to 93.70% similarity with RcbHLH130. FvbHLH130 sequence was more closely related to Rosaceae bHLH proteins and was homologous to AtFBH4. Agrobacterium-mediated transformation of Arabidopsis thaliana showed that the FvbHLH130 overexpression lines flowered seven days earlier than the control plants,and activated the expression of flowering-related genes AtAP1AtFTAtFUL and AtCO. The yeast two-hybrid results showed that FvbHLH130 interacted with FvARF4 and FvARF6,which may form protein complexes in the regulation of flowering.

Key words: Fragaria vesca, bHLH transcription factor, FvbHLH130, flowering

CLC Number: