Identification of the Targets of miR164b and Their Response to Cucumber green mottle mosaic virus Infection in Watermelon

doi:10.16420/j.issn.0513-353x.2017-0369

ACTA HORTICULTURAE SINICA ›› 2018, Vol. 45 ›› Issue (3): 482-492.doi: 10.16420/j.issn.0513-353x.2017-0369

• Research Papers • Previous Articles Next Articles

Identification of the Targets of miR164b and Their Response to Cucumber green mottle mosaic virus Infection in Watermelon

SUN Yuyan，HE Yanjun，NIU Xiaowei，CUI Di，and FAN Min*

（Institute of Vegetables，Zhejiang Academy of Agricultural Sciences，Hangzhou 310021，China）

Online:2018-03-25 Published:2018-03-25

Abstract

Abstract: The precursor gene of miR164b，Pre-miR164b，was obtained through blast of miR164b and the watermelon genome. Pre-miR164b，with a length of 97 bp，was able to form the stem-loop structure. The promoter of Pre-miR164b contains several cis-acting regulatory elements，such as light responsive element，gibberellin responsive element，ethylene responsive element，salicylic acid responsive element，and fungal elicitor responsive element. Four target genes of miR164b，annotated as NAC transcription factor，were predicted and the cleavage site was located at the 10th of miR164b at the 5′ end. Expression patterns of miR164b and NAC were different during the process of Cucumber green mottle mosaic virus（CGMMV）infection. Expression of Cla018596 was negatively correlated with miR164b. However，the expression of Cla019099，Cla023219 and Cla023357 was not correlated with miR164b.

Key words: watermelon, miR164, NAC, degradome sequencing, expression pattern

CLC Number:

S 651

SUN Yuyan，HE Yanjun，NIU Xiaowei，CUI Di，and FAN Min*. Identification of the Targets of miR164b and Their Response to Cucumber green mottle mosaic virus Infection in Watermelon[J]. ACTA HORTICULTURAE SINICA, 2018, 45(3): 482-492.

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Identification of the Targets of miR164b and Their Response to Cucumber green mottle mosaic virus Infection in Watermelon

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