Abstract:

Melon yellow spot virus disease caused by（MYSV）Melon yellow spot virus was an newly reported viral disease harm greenhouse melon production in recent years. In this study，three sets of specific primers were designed based on the conserved region of the N gene of MYSV，The reaction system was screened，optimized，and one feasible set of primers was selected to suitable for the RT-LAMP reaction. MYSV RTLAMP optimal reaction parameters were obtained in this study. The established RT-LAMP method was specific in detecting MYSV and can be accomplished in 45 min with highly advantage in sensitivity，100 times than RT-PCR. The method is suitable for rapid and accurate detection and identification of MYSV.

Key words: Melon yellow spot virus（MYSV）, RT-LAMP, rapid detection