Identification and Expression Analysis of MADS-box Genes Related to Female and Male Flowers Development in Chestnut

doi:10.16420/j.issn.0513-353x.2016-0094

ACTA HORTICULTURAE SINICA ›› 2016, Vol. 43 ›› Issue (8): 1593-1604.doi: 10.16420/j.issn.0513-353x.2016-0094

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Identification and Expression Analysis of MADS-box Genes Related to Female and Male Flowers Development in Chestnut

QIU Wen-ming，XU Yu-hai^*，and HE Xiu-juan

（Institute of Fruit and Tea，Hubei Academy of Agricultural Sciences，Fruit and Tea Subcenter of Hubei Innovation Center of Agricultural Science and Technology，Wuhan 430064，China）

Online:2016-08-25 Published:2016-08-25

Abstract

Abstract:

To screen key genes involved in the differentiation and development of flowers in chestnut （Castanea mollissima Blume），17 MADS-box genes were identified from the differentially expressed genes（DEGs）between female and male flowers of the mixed inflorescence by RNA-seq analysis. Gene structure analysis showed that two genes were Ⅰ-type MADS box genes as lack of semi-conserved K motif，while the other fifteen genes belonged to Ⅱ-type MADS-box group. And phylogenetic analysis demonstrated that among the 13 MADS-box genes with complete open reading frames（ORFs），there were two B-class genes，one C/D-class gene and three E-class genes based on the ABCDE model hypothesis. Furthermore，quantitative real time PCR was used to investigate the expression profiling of these genes in young leaves，male inflorescences at early stage，male and female flowers in the mixed inflorescence. And for one thing the results confirmed that these genes were DEGs（fold change > 2），for another it indicated that some of these MADS-box genes showed very similar expression pattern. And it assumed that these MADS-box genes might play important roles in the differentiation and development of flowers in chestnut.

Key words: chestnut, mixed inflorescence, MADS-box genes

CLC Number:

S 664.2

QIU Wen-ming，XU Yu-hai，and HE Xiu-juan. Identification and Expression Analysis of MADS-box Genes Related to Female and Male Flowers Development in Chestnut[J]. ACTA HORTICULTURAE SINICA, 2016, 43(8): 1593-1604.

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Identification and Expression Analysis of MADS-box Genes Related to Female and Male Flowers Development in Chestnut

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