Abstract: Different species，different tissues，different development periods of flowers and different hybrids of bulb of Lycoris Herb were taken as materials in this study. The mRNA expression of six housekeeping genes such as Actin，EF-1α，GAPDH，5S rRNA，Ubiquitin and β-Tubulin was detected by qRT-PCR. The stability of expression of the six reference genes were evaluated by geNorm，NormFinder，BestKeeper and Reffinder respectively. The results showed that mRNA expression of the reference genes had significant differences during the species and intraspecific in Lycoris. When analyzing the mRNA expression of different organs in Lycoris sprengeri，β-Tubulin，Actin and GAPDH could be reference genes. In the meantime，5S rRNA，EF-1α，Actin and β-Tubulin could be uesed as reference genes in different development periods of flowers. However，5S rRNA，Ubiquitin，EF-1α and β-Tubulin were best choices in different organs of Lycoris chinensis Traub. Meanwhile，Ubiquitin，β-Tubulin were suitable reference genes in different florescence. For Lycoris radiata，the stable mRNA expression in different organs β-Tubulin and 5S rRNA were appropriate，and Ubiquitin，β-Tubulin and 5S rRNA should be chosen indifferent florescence. While β-Tubulin and Actin should be apply as the reference in different hybrids bulbs. All these results will provide appropriate reference genes for further study，help achieve standardization，decrease experimental errors and increase the reliability of experimental results.

Key words: Lycoris, quantitative real-time PCR, reference gene