Construction and Characterization of a Bacterial Artificial Chromosome Library of Potato Cultivar C88

doi:10.16420/j.issn.0513-353x.2014-0885

ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (2): 361-366.doi: 10.16420/j.issn.0513-353x.2014-0885

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Construction and Characterization of a Bacterial Artificial Chromosome Library of Potato Cultivar C88

YANG Yu1，YANG Xiao-hui1,*，LI Can-hui2，GUO Xiao1，SHAN Wei-wei1，MA Wei-qing1,**，HUANG San-wen3，and LI Guang-cun1,3,**

1Institute of Vegetables and Flowers，Shandong Academy of Agricultural Sciences，Molecular Biology Key Laboratory of Shandong Facility Vegetable，Ji’nan 250100，China；2College of Life-Sciences，Yunnan Normal University，Kunming 650500，China；3Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China

Online:2015-02-25 Published:2015-02-25

Abstract

Abstract: A bacterial artificial chromosome library of potato（Solanum tuberosum L.）was constructed for‘C88’，a tetraploid potato cultivar conferring resistance to late blight，PVX and PVY，using BAC vector CopyControlTM pCC1. Fresh leaf tissues from 12–15 day-old seedlings were used to isolate high molecular weight genomic DNA，and 100–300 kb partially HindⅢ digested DNA fragments were selected and ligated into the HindⅢ site of pCC1. The library consists of 850 000 clones and stored in 1 700 pools. The average insert size of BAC clones is about 90 kb and the empty clones consist of about 2.08%. The library is equivalent to 85 haploid potato genomes. Six randomly picked clones showed stable HindⅢ fingerprints after 5 days’ successive culture，indicating that the clones in the library are stable. The library will facilitate gene cloning，sequencing and comparative genomics research of potato.

Key words: potato, bacterial artificial chromosome library, pathogen resistance

CLC Number:

S 532

YANG Yu1，YANG Xiao-hui1,*，LI Can-hui2，GUO Xiao1，SHAN Wei-wei1，MA Wei-qing1,**，HUANG San-wen3，and LI Guang-cun1,3,**. Construction and Characterization of a Bacterial Artificial Chromosome Library of Potato Cultivar C88[J]. ACTA HORTICULTURAE SINICA, 2015, 42(2): 361-366.

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Construction and Characterization of a Bacterial Artificial Chromosome Library of Potato Cultivar C88

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