The Effect of Silencing Chalcone Synthase on Anthocyanin Metabolism in Peach

doi:10.16420/j.issn.0513-353x.2014-0554

ACTA HORTICULTURAE SINICA ›› 2015, Vol. 42 ›› Issue (1): 31-37.doi: 10.16420/j.issn.0513-353x.2014-0554

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The Effect of Silencing Chalcone Synthase on Anthocyanin Metabolism in Peach

ZHANG Lei, ZHU Li-Xin, XU Chuan, CUI Chun-Mei, SHENG Hong-Ya, LI Rui, WANG Hong-Qing

College of Agronomy and Biotechnology，China Agricultural University，Beijing 100193，China

Online:2015-01-25 Published:2015-01-25

Abstract

Abstract: In this study，we cloned a 493 bp CHS fragment from the peach（Prunus persica）peal and constructed interference vector TRV-LIC-CHSi using virus-induced gene silencing（VIGS）to study the function of PpCHS in anthocyanin pathway in peach skin by Agrobacterium infiltration. The interference successfully decreased CHS expression by 88.8% and remarkably modified anthocyanins content in peach skin，specifically，anthocyanin content and quercetin content decreased by 87% and 35% respectively；Chlorogenic acid content increased by 57% up；The content of procyanidin，catechin and kaempferol did not show significant change. Our results demonstrated that the whole anthocyanin pathway was turned to chlorogenic acid and its complexes synthesis when the expression of CHS was severely repressed. CHS is an important gene in anthocyanin synthesis and regulates the relative amount of specific flavonoids.

Key words: peach, chalcone synthase（CHS）, virus-induced gene silencing（VIGS）, anthocyanin, chlorogenic acid

CLC Number:

S 662.1

ZHANG Lei, ZHU Li-Xin, XU Chuan, CUI Chun-Mei, SHENG Hong-Ya, LI Rui, WANG Hong-Qing. The Effect of Silencing Chalcone Synthase on Anthocyanin Metabolism in Peach[J]. ACTA HORTICULTURAE SINICA, 2015, 42(1): 31-37.

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The Effect of Silencing Chalcone Synthase on Anthocyanin Metabolism in Peach

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