https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (7): 1476-1483.

Previous Articles     Next Articles

Preliminary Screening of Dormancy Regulation Related Genes from SSH
Library Constructed Using Dormant and Dormancy Breaking Bulbs of
Lilium pumilum

LIU Fang1,2,WANG Jia-yan1,and ZHOU Yun-wei1,*   

  1. 1College of Landscape Architecture,Northeast Forestry University,Harbin 150040,China;2College of Agriculture,
    Heilongjiang Bayi Agricultural University,Daqing,Heilongjiang 163319,China
  • Online:2014-07-25 Published:2014-07-25

Abstract: In order to verify the differences of gene expression in the process of lily bulb dormancy
breaking,two SSH-cDNA libraries were constructed using dormant and dormancy breaking bulbs. The
forward library contained genes preferentially expressed in dormancy breaking bulbs and the reverse
library contained genes preferentially expressed in dormant bulbs. The results showed that differential
express genes were enriched effectively in two libraries,and subtraction efficiency reached the demand.
Most of the length of inserted fragments was 250–1 000 bp. One hundred positive clones were randomly
selected for sequencing from the two libraries. We analyzed ESTs in BLASTx of NCBI and unigenes
positioning in the pathways using KOBAS system. Results indicated that in dormant bulb library,these
functionally known genes were detected in stress response. Phenylalanine metabolism and MAPK
signaling pathway were associated with dormancy. In bulb dormancy breaking library,the function of ESTs involved in sugar metabolism,hormone response and signal transduction,linoleic acid metabolism
and starch and sucrose metabolic pathway was involved in dormancy breaking process.

Key words: Lilium pumilum, bulb, dormancy, suppression subtractive hybridization

CLC Number: