Molecular Identification and Cold-resistance Analysis of the AmEBP1 Transgenic Apricot Plants

ACTA HORTICULTURAE SINICA ›› 2014, Vol. 41 ›› Issue (5): 825-832.

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Molecular Identification and Cold-resistance Analysis of the AmEBP1 Transgenic Apricot Plants

NIU Qing-Lin, CAO Bang-Hua, WANG Yu-Shan, LIU Jing, ZHAO Jin-Hong, LUO Lei, HUANG Yan-Yan, FENG Dian-Qi

NIU Qing-lin1，CAO Bang-hua1,*，WANG Yu-shan2，LIU Jing2，ZHAO Jin-hong2，LUO Lei2，HUANG Yan-yan2，and FENG Dian-qi1,2,*

Online:2014-05-25 Published:2014-05-25

Abstract

Abstract: The AmEBP1 gene coming from Ammopiptanthus mongolicus，was transformed into the
immature embryo of apricot by pollen tube. Grew in the modified WPM media，five transgenic lines were
obtained based on the following selections including kanamycin resistance，PCR and Southern analyses.
Results of the cold resistance tests pointed out that in the low temperature treatments（–4 and–8 ℃），the
transgenic plants exhibited higher survival rates than the untransformed control plants. Under the lower
temperature cold treatment，the REC and MDA of transgenic plants was obviously lower than that of
untransformed control plants. The LT50 of transgenic lines was remarkable lower than that of the
untransformed control plants by 2.13 ℃. These results indicate that the imported AmEBP1 gene increased
the cold resistance of the Daguo apricot.

Key words: apricot, pollen tube, AmEBP1 gene, gene transformation

CLC Number:

S 662.2

NIU Qing-Lin, CAO Bang-Hua, WANG Yu-Shan, LIU Jing, ZHAO Jin-Hong, LUO Lei, HUANG Yan-Yan, FENG Dian-Qi. Molecular Identification and Cold-resistance Analysis of the AmEBP1 Transgenic Apricot Plants[J]. ACTA HORTICULTURAE SINICA, 2014, 41(5): 825-832.

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Molecular Identification and Cold-resistance Analysis of the AmEBP1 Transgenic Apricot Plants

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