Abstract: In this study，we optimized the High-performance liquid chromatography（HPLC）determination system for phenolic compounds in peach，and determined and compared phenolic compounds in three types of color peach flesh，i.e. blood，yellow and white. The phenolic compounds of peach flesh were extracted with ethanol（0.1% H3PO4）via ZORBAX SB-C18 column（4.6 mm × 250 mm，5 μm），by using ethanol（0.1% H3PO4）and water（0.1% H3PO4）as solvents at the flow rate of 1.0 mL · min-1 under the temperature of 30 ℃ and UV of 280 nm. This method was particularly suitable for the determination of phenolic compounds of peach flesh. The results showed that chlorogenic acid，neochlorogenic acid，catechin，epicatechin，rutin，quercetin，gallic acid，ferulic acid，phlorizin and phloretin were detected in peach flesh. The main components and content of phenolic compounds were different in the three types of flesh color peaches. In details，epicatechin，chlorogenic acid，catechin and neochlorogenic acid were the main components in blood flesh peach. Neochlorogenic acid，chlorogenic acid and catechin were the first most components in yellow flesh peach，while neochlorogenic acid，catechin and rutin in white flesh peach. Epicatechin and neochlorogenic acid were the highest phenolic compounds in blood flesh peach and yellow flesh peach，respectively，with the content of 78.91–673.90 mg · kg-1FW and 7.28–25.57 mg · kg-1FW. However，the phenolic compounds in white flesh peach was distinct among cultivars，and neochlorogenic acid（3.17–6.16 mg · kg-1FW）and catechin（4.21–14.55 mg · kg-1FW）contents were higher than the other components. In particular，the contents of phenolic compounds varied significantly in different color flesh peaches. As the main phenolic compounds in peach flesh，chlorogenic acid，neochlorogenic acid，catechin，epicatechin and rutin were significant higher in blood flesh peach than that of white and yellow flesh peach. In addition，the content of some phenolic compounds was significantly different among cultivars within the same color flesh，which may be favorably used to screen specific germplasms in peach.

Key words: peach, flesh color, phenolic compounds, high-performance liquid chromatography