Screening and Identification of Genes Involved in Regulating Internode Length for Semi-dwarf Peach

ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (5): 943-.

• Research Notes • Previous Articles Next Articles

Screening and Identification of Genes Involved in Regulating Internode Length for Semi-dwarf Peach

LU Zhen-Hua, LI Yu-Ting, WANG Zhi-Qiang-*, NIU Liang, CUI Guo-Chao, WANG Yu-Lan

（Zhengzhou Fruit Research Institute，Chinese Academy of Agricultural Sciences，National Peach and Grape Improvement
Center，Key Laboratory of Fruit Breeding Technology of Ministry of Agriculture，Zhengzhou 450009，China）

Online:2013-05-25 Published:2013-05-25

Abstract

Abstract: This study aimed to select and identify genes related to regulate the length of internode，
and provide basis for revealing expression pattern of regulating internode length related genes and cloning
the gene of controlling semi-dwarf trait. cDNA-AFLP technique was employed to investigate differentially
expressed genes. The transcript-derived fragments（TDFs）was recycled，cloned，sequenced and functionally
annotated and then validate by qRT-PCR. A total of 9 021 TDFs were screened by 256 primer
combinations，of which 899 TDFs were identified differentially expression. Totally，497 TDFs were
successfully sequenced and 254 sequences were high similar to genes with known functions. The results
indicated that these genes were involved in signal transduction，growth and development，transcription
regulation，stress tolerance，protein degradation and synthesis and so on. The expression abundance of 26
genes validated by real-time quantitative RT-PCR was consistent with the result of cDNA-AFLP analysis.
We generated candidate genes in plant IAA，CK，BR，ABA and cell cycle regulation pathways，which will
help to elucidate the molecular regulation mechanism of internode length.

Key words: peach, semi-dwarf type, cDNA-AFLP, differentially expressed genes, internode length, function annotation

CLC Number:

S 662.1

LU Zhen-Hua, LI Yu-Ting, WANG Zhi-Qiang-*, NIU Liang, CUI Guo-Chao, WANG Yu-Lan. Screening and Identification of Genes Involved in Regulating Internode Length for Semi-dwarf Peach[J]. ACTA HORTICULTURAE SINICA, 2013, 40(5): 943-.

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Screening and Identification of Genes Involved in Regulating Internode Length for Semi-dwarf Peach

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