Abstract: A full length of GID1A gene was isolated from grapevine‘Fujiminori’（Vitis vinifera ×
V. labrusca）by RT-PCR and RACE（rapid amplification of cDNA ends）method. Sequence analysis
indicated that VvGID1A was 1 681 bp in full length，and an opening reading frame（ORF）of 1 035 bp
encoding 344 amino acids. Subsequently，amino acid sequence analysis revealed that VvGID1A included
two conserved domains of hormone sensitive lipase（HSL）：HGG and GXSXG. Phylogenetic analysis
showed that VvGID1A share 70.25%，70.08% and 68.21% identity with GID1 in Medicago truncatula，
Gossypium hirsutum and Arabidopsis thaliana. Real-time fluorescent quantitative PCR indicated that the
expression of VvGID1A gene in flower，old leaf and tendril is higher than that in stem tip and young leaf.
Furthermore，the expression of VvGID1A gene was lower in gibberellins treated fruit than in the control.
After construction of transient expression vector and onion epidermal cell transformation，subcellular
localization assays showed that the VvGID1A protein was located in the nucleus.

Key words: Vitis vinifera, gibberellin, VvGID1A, subcellular localization, expression level