Induction and Determination of Tetraploid Plants of Phaius tankervilleae

ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (10): 2033-2038.

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Induction and Determination of Tetraploid Plants of Phaius tankervilleae

LI Dou-dou1， LIU Zhi-long2，HUANG Ming-zhong2，CHEN Ji-tian1，TANG Yan-qiong1,*，and YANG Guang-sui2,*

（1Key Laboratory of Protection and Development Utilization of Tropical Crop Gemplasm Resources，Hainan University，Ministry of Education；College of Agronomy，Hainan University，Haikou 570228，China；2Tropical Crops Genetic Resources Institute，Chinese Academy of Tropical Agricultural Sciences，Danzhou，Hainan 571737，China）

Received:2013-07-05 Online:2013-10-25 Published:2013-10-25

Abstract

Abstract: Tetraploids of Phaius tankervilleae were induced through the treatment of protocorms with colchicine in vitro. The results showed that ploidy levels of protocorms changed with colchicine soaking and the highest induction rate of 22.5% was obtained by immersing protocorms into 0.02% colchicine solution for 6 days. Cytological determination of regenerated plants indicated that three tetraploid plantlets were obtained among mutated plants survived，and the morphology，the number and size of stomata of the mutants were different from those of the diploid plants，with obviously bigger nucleus.

Key words: Phaius tankervilleae, colchicine, tetraploid, protocorm

CLC Number:

S 682.31

LI Dou-dou1， LIU Zhi-long2，HUANG Ming-zhong2，CHEN Ji-tian1，TANG Yan-qiong1,*，and YANG Guang-sui2,*. Induction and Determination of Tetraploid Plants of Phaius tankervilleae[J]. ACTA HORTICULTURAE SINICA, 2013, 40(10): 2033-2038.

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Induction and Determination of Tetraploid Plants of Phaius tankervilleae

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