Cloning and Expression Analysis on AP1 Homologous Gene from Cymbidium ensifolium and Interaction Analysis Between AP1 and MADS-box Transcription Factors

ACTA HORTICULTURAE SINICA ›› 2013, Vol. 40 ›› Issue (10): 1935-1942.

• Ornamental Plants • Previous Articles Next Articles

Cloning and Expression Analysis on AP1 Homologous Gene from Cymbidium ensifolium and Interaction Analysis Between AP1 and MADS-box Transcription Factors

WU Jing-hua，WU Shao-hua*，YANG Chao，and ZHANG Zhi-zhong

（College of Horticulture，Fujian Agriculture and Forestry University，Fuzhou 350002，China）

Received:2013-06-26 Online:2013-10-25 Published:2013-10-25

Abstract

Abstract: To investigate the function of AP1 genes on flower development in Cymbidium ensifolium，AP1 was cloned from‘Sijidaqing’flower buds and the full length of AP1 cDNA was amplified by RACE method，named CeAP1. CeAP1 is 866 bp in length with a 726 nucleotides ORF that putatively encodes a protein with 241 amino acids. Homology analysis showed that the CeAP1 was highly homologous to the AP1MADS-box genes of orchids. Quantitative real-time PCR analysis demonstrated that CeAP1 was expressed in the initial period of floral development and vegetative organs. The yeast two-hybrid analysis indicated that the CeAP1was not only able to form homodimer but also formed heterodimers with the other MADS-box transcription factors except for CeAG1.

Key words: Cymbidium ensifolium, flower development, AP1 homologous gene, interaction

CLC Number:

S 682.31

WU Jing-hua，WU Shao-hua*，YANG Chao，and ZHANG Zhi-zhong. Cloning and Expression Analysis on AP1 Homologous Gene from Cymbidium ensifolium and Interaction Analysis Between AP1 and MADS-box Transcription Factors[J]. ACTA HORTICULTURAE SINICA, 2013, 40(10): 1935-1942.

[1]	TIAN Mingkang, XU Zhixiang, LIU Xiuqun, SUI Shunzhao, LI Mingyang, and LI Zhineng, . Identification of the AP2 Subfamily Transcription Factors in Chimonanthus praecox and the Functional Study of CpAP2-L11 [J]. Acta Horticulturae Sinica, 2023, 50(2): 382-396.
[2]	YAN Wenyuan, QIN Junhong, DUAN Shaoguang, XU Jianfei, JIAN Yinqiao, JIN Liping, LI Guangcun. The Effect of Water-nitrogen Coupling on Potato Photosynthesis,Tuber Formation and Quality [J]. Acta Horticulturae Sinica, 2022, 49(7): 1491-1504.
[3]	GAO Weilin, ZHANG Liman, XUE Chaoling, ZHANG Yao, LIU Mengjun, ZHAO Jin. Expression of E-type MADS-box Genes in Flower and Fruits and Protein Interaction Analysis in Chinese Jujube [J]. Acta Horticulturae Sinica, 2022, 49(4): 739-748.
[4]	MO Cuiping, WU Zilin, GUO Yongyi, ZHANG Shuguang, LI Pengfei, LI Huaping. The Neutral Interaction of Co-infection Papaya Between Papaya Ringspot Virus and Papaya Leaf Distortion Mosaic Virus [J]. Acta Horticulturae Sinica, 2022, 49(11): 2336-2346.
[5]	LAN Liming, LUO Changguo, WANG Sanhong. Analysis of Resistance Mechanism to Powdery Mildew Based on Transcriptome Sequencing in Malus hupehensis [J]. Acta Horticulturae Sinica, 2021, 48(5): 860-872.
[6]	ZHANG Qian, YANG Nan, SANG Haiyu, ZHAO Rong, SONG Xiaoxi, CHEN Longqing, XIANG Lin, ZHAO Kaige. Cloning and Functional Analysis of CpTT8 Related to Anthocyanin Synthesis in Wintersweet（Chimonanthus praecox） [J]. Acta Horticulturae Sinica, 2021, 48(10): 1945-1955.
[7]	ZENG Tuo, LI Jiawen, ZHOU Li, LI Jinjin, SHI Anqi, FU Hansen, LUO Jing, ZHENG Riru, WANG Yuanyuan, WANG Caiyun. Advances in the Mutualistic and Antagonistic Interactions Between Flower Colors and the Pollinators of Ornamental Plants [J]. Acta Horticulturae Sinica, 2021, 48(10): 2001-2017.
[8]	ZHANG Yan，XU Shenping，LIANG Fang，JIANG Suhua，YUAN Xiuyun，NIU Suyan，and CUI Bo*. Cloning SVP Family Gene PhSVP from Phalaenopsis and Its Expression Analysis During Flower Development [J]. ACTA HORTICULTURAE SINICA, 2020, 47(6): 1111-1125.
[9]	LIN Lu, LI Zhiqiang, YU Lu, WANG Hongning, and NIU Zimian, . Photosynthetic Responses to Interaction of Light Intensity and CO2 Concentration and Photoinhibition Characteristics of Two Apple Canopy Shapes [J]. Acta Horticulturae Sinica, 2020, 47(11): 2073-2085.
[10]	XIANG Yuanping, WANG Yidan, HE Hongjun, and XU Qijiang, . Plant Transposable Elements and the Effects of Insertion Mutations on Flower Development in Horticultural Plants [J]. Acta Horticulturae Sinica, 2020, 47(11): 2247-2266.
[11]	LI Chengru, DONG Na, LI Xiaoping, WU Shasha, LIU Zhongjian, and ZHAI Junwen. A Review of MADS-box Genes，the Molecular Regulatory Genes for Floral Organ Development in Orchidaceae [J]. Acta Horticulturae Sinica, 2020, 47(10): 2047-2062.
[12]	WU Jinghua，YANG Chao，and WU Shaohua*. Expression and Function Analysis of AGL6 Homologous Genes in Cymbidium ensifolium [J]. ACTA HORTICULTURAE SINICA, 2019, 46(6): 1123-1133.
[13]	GAO Qiguo1,，LEI Zhenze1,，LIANG Yunfei1,*，JIANG Yupeng1，and ZHU Liquan2. Screening of Brassica oleracea MLPK-interacting PUB Proteins Based on Gene Family Analysis [J]. ACTA HORTICULTURAE SINICA, 2019, 46(4): 714-722.
[14]	WANG Yu，JIANG Wei，YAN Kai，ZHOU Wenwen，WANG Zhimin，WEI Dayong，and TANG Qinglin. Protein Interactions of Flowering Inhibitors AGL18 and HDA9 with Integrator Factors in Brassica oleracea var. italica [J]. ACTA HORTICULTURAE SINICA, 2018, 45(12): 2383-2394.
[15]	MA Jing，MEN Weiting，CHEN Xinli，SUI Shunzhao，and LI Mingyang*. Cloning and Expression Analysis of a Small GTP-binding Protein Gene（CpRAC1）in Chimonanthus praecox [J]. ACTA HORTICULTURAE SINICA, 2018, 45(11): 2177-2187.

Cloning and Expression Analysis on AP1 Homologous Gene from Cymbidium ensifolium and Interaction Analysis Between AP1 and MADS-box Transcription Factors

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments