Screening of Molecular Markers Linked to Cladosporium fulvum Resistant Gene Cf12 in Tomato and Identification of Germplasm Resource

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (5): 985-991.

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Screening of Molecular Markers Linked to Cladosporium fulvum Resistant Gene Cf12 in Tomato and Identification of Germplasm Resource

ZHAO Ting-Ting, SONG Ning-Ning, JIANG Jing-Bin, ZHANG He, KANG Li-Gong, LI Jing-Fu, XU Xiang-Yang

（College of horticulture，Northeast Agricultural University，Harbin 150030，China）

Online:2012-05-25 Published:2012-05-25

Abstract

Abstract: The study identified the molecular marker linked to Cladosporium fulvum resistant gene Cf12 in tomato. The linked marker was screened with a F₂ population between a resistant and a susceptible parent（05HN36 × 051355）by AFLP technology. Six AFLP markers had been identified to linked with Cf12 gene：E86M51-C，E46M50，E95M59-D，E35M81，E78M36-C and E47M50-C. The genetic distances were 5.1，8.9，9.1，9.2，10.2 and 10.8 cM respectively. The AFLP marker E86M51-C was converted into SCAR marker. We obtained 16 cultivars which contained this marker. These cultivars could be used in C. fulvum resistant breeding practice.

Key words: tomato, Cladosporium fulvum, resistance gene Cf12, AFLP, SCAR

ZHAO Ting-Ting, SONG Ning-Ning, JIANG Jing-Bin, ZHANG He, KANG Li-Gong, LI Jing-Fu, XU Xiang-Yang. Screening of Molecular Markers Linked to Cladosporium fulvum Resistant Gene Cf12 in Tomato and Identification of Germplasm Resource[J]. ACTA HORTICULTURAE SINICA, 2012, 39(5): 985-991.

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Screening of Molecular Markers Linked to Cladosporium fulvum Resistant Gene Cf12 in Tomato and Identification of Germplasm Resource

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