The Proteinase Activity and Types of Osmanthus fragrans Petals During Flower Opening and Senescence

ACTA HORTICULTURAE SINICA ›› 2012, Vol. 39 ›› Issue (10): 1967-1974.

• Ornamental Plants • Previous Articles Next Articles

The Proteinase Activity and Types of Osmanthus fragrans Petals During Flower Opening and Senescence

YANG Shao-Kun, CAI Xuan, ZOU Jing-Jing, WANG Cai-Yun

（Key Laboratory of Horticultural Plant Biology，Ministry of Education，College of Horticulture and Forestry，Huazhong Agricultural University，Wuhan 430070，China）

Online:2012-10-25 Published:2012-10-25

Abstract

Abstract: The contents of soluble protein and free amino acid，the changes of proteinase activity and types of Osmanthus fragrans‘Liuye Jingui’were determined during flower opening and development stages from linggeng stage，initial flowering stage，full flowering stage，late full flowering stage to wilting stage. The results showed that variation patterns between soluble protein and free amino acid were different. The optimum temperature and pH for protease measure were 37 ℃ and pH 8.0 respectively. The petal proteinase activity which was detected by spectrophotometry and gel-SDS-PAGE electrophoresis increased continuously until late full flowering stage，and then sharply decreased at wilting stage. Three kinds of proteinases，serine protease，cystein protease and metallo protease，were found by using proteinase inhibitors. Serine protease，accounted for about 65%–75% in total，was the most active one among the three proteases to influence petal senescence of sweet osmanthus.

Key words: Osmanthus fragrans, flower opening, senescence, proteinase, gel-SDS-PAGE

CLC Number:

S 685.13

YANG Shao-Kun, CAI Xuan, ZOU Jing-Jing, WANG Cai-Yun. The Proteinase Activity and Types of Osmanthus fragrans Petals During Flower Opening and Senescence[J]. ACTA HORTICULTURAE SINICA, 2012, 39(10): 1967-1974.

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The Proteinase Activity and Types of Osmanthus fragrans Petals During Flower Opening and Senescence

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