Abstract: Sixty-four cherry（Prunus pseudocerasus L.）accessions from Guizhou Province，as well as the cultivar Black Pearl，were analyzed by ISSR markers. A total of 228 markers were scored from the 21 primers，which were screened out from 90 primers and demonstrated highly reproducible，clear and polymorphic bands. Among the obtained markers，199 were polymorphic，accounting for 87.28% of the total. Analyzed by NTSYS2.01，the genetic similarity coefficient among the accessions ranged from 0.49 to 0.90，reflecting the high genetic diversity of cherry germplasms in Guizhou Province. The UPGMA dendrogram demonstrated that the accessions from Bijie，Zunyi and Qiannan areas were genetically more diversified. In particular the accessions from Bijie exhibited the highest degree of diversity in fruit quality，which may be used as elite materials for further genetic improvement in this species.

Key words: cherry, germplasm, ISSR, genetic diversity