Studies on Tetraploid Breeding Techniques by Using Isolated Microspore Culture Techniques in Brassica campestris ssp. chinensis（L.）Makino

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (7): 1299-1307.

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Studies on Tetraploid Breeding Techniques by Using Isolated Microspore Culture Techniques in Brassica campestris ssp. chinensis（L.）Makino

FENG Hui*，FU Ying，YANG Shuo，BAO Mei-li，and LIU Xue-li

（College of Horticulture，Shenyang Agricultural University，Liaoning Province Key Laboratory of Genetics and Breeding of Cruciferous Vegetable Crops，Shenyang 110866，China）

Received:2011-03-30 Revised:2011-05-26 Online:2011-07-25 Published:2011-07-25
Contact: FENG Hui*

Abstract

Abstract: The aim of this study was to develop diversified tetraploid lines in Brassica campestris ssp. chinensis（L.）Makino and thereby facilitate heterosis breeding. Sixteen hybrid varieties were used for isolated microspore culture and 393 regenerated plants were obtained. Among these，113 plants were identified as tetraploids based on morphological identification，examination of stomatal characteristics，size measurement and viability testing of pollen，and chromosome counts of the pollen mother cells. Furthermore，3 tetraploid lines showing excellent characteristics were screened. The 3 selected tetraploid lines were used as parents，and a tetraploid hybrid‘T24 × T11’with significant heterosis and comprehensive quality traits was selected through a variety comparison tests. A technical system for tetraploid heterosis breeding was established based on isolated microspore in Brassica campestris ssp. chinensis（L.）Makino.

Key words: Brassica campestris ssp. chinensis（L.）Makino, isolated microspore culture, tetraploid, heterosis breeding

CLC Number:

S 634.3

FENG Hui;FU Ying;YANG Shuo;BAO Mei-li;and LIU Xue-li . Studies on Tetraploid Breeding Techniques by Using Isolated Microspore Culture Techniques in Brassica campestris ssp. chinensis（L.）Makino [J]. ACTA HORTICULTURAE SINICA, 2011, 38(7): 1299-1307.

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Studies on Tetraploid Breeding Techniques by Using Isolated Microspore Culture Techniques in Brassica campestris ssp. chinensis（L.）Makino

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