https://www.ahs.ac.cn/images/0513-353X/images/top-banner1.jpg|#|苹果
https://www.ahs.ac.cn/images/0513-353X/images/top-banner2.jpg|#|甘蓝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner3.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner4.jpg|#|灵芝
https://www.ahs.ac.cn/images/0513-353X/images/top-banner5.jpg|#|桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner6.jpg|#|黄瓜
https://www.ahs.ac.cn/images/0513-353X/images/top-banner7.jpg|#|蝴蝶兰
https://www.ahs.ac.cn/images/0513-353X/images/top-banner8.jpg|#|樱桃
https://www.ahs.ac.cn/images/0513-353X/images/top-banner9.jpg|#|观赏荷花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner10.jpg|#|菊花
https://www.ahs.ac.cn/images/0513-353X/images/top-banner11.jpg|#|月季
https://www.ahs.ac.cn/images/0513-353X/images/top-banner12.jpg|#|菊花

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (6): 1197-1204.

• Technologies and Methods • Previous Articles     Next Articles

Establishment of Detection System for Soluble Acid Invertase Activity in Lilium davidii var. unicolor Bulb

SUN Hong-mei*,HE Ling,WANG Wei-wei,WANG Chun-xia,and LI Tian-lai   

  1. (College of Horticulture,Shenyang Agricultural University,Horticulture Key Laboratory of Ministry of Education,Key Laboratory of Protected Horticulture of Liaoning Province,Shenyang 110866,China)
  • Received:2010-12-21 Revised:2011-05-11 Online:2011-06-25 Published:2011-06-25
  • Contact: SUN Hong-mei

Abstract: In order to make a thorough study in sucrose-metabolizing mechanism of lily bulblet,the exterior scales of Lilium davidii var. unicolor bulb were used in present study to explore the effects of buffers with different sorts and pH values,reaction durations and reaction temperatures on SAI activity. Then SAI activity detection system was established. The results indicated that the optimal extraction and reaction buffers were Hepes-NaOH(pH 8.0)and HAc-K3PO4(pH 4.8),respectively. And SAI has an optimum reaction duration and temperature of 30 min and 40 ℃.

Key words: lily, bulb, soluble acid invertase, extraction condition, reaction condition

CLC Number: