Abstract: The ploidy level，pollen morphology，and pollen germination of five Asiatic lilies，‘Renoir’，‘Eleganza’，‘Pollyanna’，‘Brunello’and‘Val di Sole’，were investigated in the present paper. The results show：1. They have different ploidy levels.‘Renoir’，‘Eleganza’and‘Pollyanna’are diploid （2n = 2x = 24），‘Brunello’and‘Val di Sole’are tetraploid（2n = 4x = 48）；2. All the cultivars have two classes of pollen，i. e.，normal stainable elliptical pollen and abnormal unstainable wrinkled pollen；3. The tetraploid‘Brunello’had 80% pollen germination rate，which is much higher than others ranged from 24%–36%；4. By embryo rescue，18 seedling lines were obtained from 4x–2x of six interploid crossing combinations. The analyzed 12 lines out of them are triploid（2n = 3x = 36）based on chromosome preparation. Since triploid lilies have the advantages over diploid and lily can be propagated vegetatively， it would be highly possible to select new cultivars from the triploid seedlings.

Key words: Asiatic lily, interploid hybridization, triploid, embryo rescue