Fine Mapping of the Root-knot Nematode Resistance Gene Me3 in Pepper

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (2): 288-288–294.

• Vegetables • Previous Articles Next Articles

Fine Mapping of the Root-knot Nematode Resistance Gene Me3 in Pepper

XU Xiao-yan1,2，LIU Feng1,2，KANG Hou-xiang1，ZHANG Zhu-qing2，ZOU Xue-xiao2,*，and XIE Bing-yan1,*

（1Institute of Vegetables and Flowers，Chinese Academy of Agricultural Sciences，Beijing 100081，China；2Institute of Vegetable，Hunan Academy of Agricultural Sciences，Changsha 410128，China）

Received:2010-12-16 Revised:2011-01-21 Online:2011-02-25 Published:2011-02-25
Contact: ZOU Xue-xiao and XIE Bing-yan

Abstract

Abstract: The pepper（Capsicum annuum）resistant inbred line‘HDA149’with Me3 was used as male parent，and the susceptible inbred line‘8214’was used as female parent in the study. Mapping population included 2 133 F2 individuals. Bulked-segregant analysis with DNA pools，from susceptible or resistant F2 progenies，was performed to identify markers linked to Me3. There were 285 pairs of primers with polymorphism between two parents，but just SSCP_B322 and EPMS658 were polymorphic between the two bulks. Then F2 individuals were amplified with the two pairs of primers，recording data. Linkage analysis used JoinMap 3.0 software. The genetic distance of SSCP_B322 and EPMS658 was 0.56 cM and 1.33 cM respectively，and two markers were two sides of Me3 gene. They had been verified in BC1 group and F3 families，which has been practically useful to marker-assisted selection in pepper breeding and Me3 gene cloning.

Key words: pepper, Capsicum annuum L., Meloidogyne incognita, Me3 gene, fine map

CLC Number:

S 641.3

XU Xiao-yan;LIU Feng;KANG Hou-xiang;ZHANG Zhu-qing;ZOU Xue-xiao;and XIE Bing-yan;. Fine Mapping of the Root-knot Nematode Resistance Gene Me3 in Pepper[J]. ACTA HORTICULTURAE SINICA, 2011, 38(2): 288-288–294.

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Fine Mapping of the Root-knot Nematode Resistance Gene Me3 in Pepper

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