Abstract: The ARC1 cDNA sequence was isolated from stigma of ornamental kale（Brassica oleracea var. acephala）S 13-b S 13-b homozygotes using RT-PCR techniques，named BoARC1（GenBank accession No. EU344909）. The BoARC1 cDNA sequence had an ORF of 1 992 bp，encoding a 663-aa protein. The deduced amino acid sequence of isolated BoARC1 shared 94% identity with Brassica napus ARC1. Southern blot analysis showed that there was a single copy of BoARC1 gene in genome. Northern blot analysis showed that BoARC1 mRNA only expressed in the stigma. The yeast two-hybrid analysis indicated that the BoARC1 interacted with SRK3，SRK13-b and SRK910.

Key words: Brassica oleracea var. acephala, self-incompatibility, ARC1, interaction