Abstract: With seedling inoculation in greenhouse，progenies from interspecific cross between cultivated cucumber and sour cucumber were screened and an introgression line ILs-10-1 was identified with resistance to the root-knot nematode Meloidogyne incognita following resistance evaluation，morphological observation and molecular characterization. The results showed that the ILs-10-1 displayed stable resistance to M. incognita. In morphology，ILs-10-1 was between its parents and characterized with smaller leaf，short fruit，brown spine，multi-branching. One pair（SSR18648）out of 302 pairs of SSR primers could repeatedly amplify special DNA fragment from its wild parent of sour cucumber，confirming its nature of interspecific progenies from cross between cultivated cucumber and sour cucumber at molecular level.

Key words: cucumber, introgression line, Meloidogyne incognita, morphological identification, SSR marker