New Alien Additon Lines Resistance to Black Rot Generated by Somatic Hybridization Between Cauliflower and Black Mustard

ACTA HORTICULTURAE SINICA ›› 2011, Vol. 38 ›› Issue (10): 1901-1910.

• Vegetables • Previous Articles Next Articles

New Alien Additon Lines Resistance to Black Rot Generated by Somatic Hybridization Between Cauliflower and Black Mustard

WANG Gui-xiang1，YAN Hong2，ZENG Xing-ying1，SHENG Xiao-guang1，TANG Yu1，HAN Shuo1，ZONG Mei1，LU Kun1，and LIU Fan1,*

1Beijing Vegetable Research Center，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100097，China；2Institute of Plant and Environmental Protection，Beijing Academy of Agriculture and Forestry Sciences，Beijing 100097，China

Received:2011-08-18 Revised:2011-10-08 Online:2011-10-25 Published:2011-10-25
Contact: LIU Fan1,*

Abstract

Abstract: In this study，the asymmetrical somatic hybrids of cauliflower and Brassica nigra and their selfed or backcrossed progenies were analysed using morphological，cytological and molecular methods. Fifty advanced selfing or backcrossing descendants were obtained. Besides 30 lines with an intermediate morphology to that of the parents，there were 20 lines with a nuclear DNA content below 2.24 pg · cell-1 and chromosomes number less than 28 displaying the cauliflower-like morphology. Combining with successive black rot resistance assay，a set of alien additon lines were obtained，in which 12 lines with high resistance，17 lines being resistant，those accounted for 14% of the total materials tested. Three plants（PFCN29 BC3-3-5，PFCN16-1 S2BC3-107 and PFCN14-1 S1BC4-123）displayed a more cauliflower-like morphology and high resistance to Xcc strain CH15 were confirmed to have a total set of cauliflower chromosomes added with 2，7 and 8 B. nigra-derived chromosomes respectively by genomic in situ hybridization（GISH）.

Key words: cauliflower, black mustard, somatic hybridization, black rot, alien additon line

CLC Number:

S 635.3
S 63

WANG Gui-xiang;YAN Hong;ZENG Xing-ying;SHENG Xiao-guang;TANG Yu;HAN Shuo;ZONG Mei;LU Kun;and LIU Fan;. New Alien Additon Lines Resistance to Black Rot Generated by Somatic Hybridization Between Cauliflower and Black Mustard [J]. ACTA HORTICULTURAE SINICA, 2011, 38(10): 1901-1910.

[1]	WANG Mengmeng, SUN Deling, CHEN Rui, YANG Yingxia, ZHANG Guan, LÜ Mingjie, WANG Qian, XIE Tianyu, NIU Guobao, SHAN Xiaozheng, TAN Jin, and YAO Xingwei, . Construction and Evaluation of Cauliflower Core Collection [J]. Acta Horticulturae Sinica, 2023, 50(2): 421-431.
[2]	ZHANG Shuangshuang, SU Wei, LIU Yang, WANG Haiping, SONG Jiangping, YANG Wenlong, JIA Huixia, Zhang Xiaohui, LI Xixiang. Germplasm Innovation and Black Rot Resistance Transferring in Brassica rapa ssp. chinensis Through Interspecific Hybridization with Brassica carinata [J]. Acta Horticulturae Sinica, 2021, 48(7): 1304-1316.
[3]	ZHU Shiyang, ZHANG Xiaoling, LIU Qing, ZHONG Weijie, LUO Tiankuan, TANG Zheng, XU Qian, ZHOU Yuanchang. Cytoplasmic Effect Analysis of Different CMS Sources of Cauliflower [J]. Acta Horticulturae Sinica, 2021, 48(6): 1107-1122.
[4]	ZHANG Xiaoling, LIU Qing, ZHU Shiyang, ZHONG Weijie, LUO Tiankuan, TANG Zheng, and QIU Boyin. A New Loose-curd Cauliflower Hybrid‘Ousong 60’ [J]. Acta Horticulturae Sinica, 2020, 47(9): 1861-1862.
[5]	YAO Xingwei，NIU Guobao，LIU Lili，SHAN Xiaozheng，WEN Zhenghua，JIANG Hanmin，ZHANG Xiaoli，and SUN Deling*. A New Cauliflower Cultivar‘Jinpin 75’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(S2): 2783-2784.
[6]	LIU Qing，ZHANG Xiaoling*，ZHU Shiyang，TANG Zheng，Qiu Boyin，and LUO Tiankuan. A New Loose-curd Cauliflower Cultivar‘Ousong 90 d’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(S2): 2785-2786.
[7]	YANG Long，LI Yirong，SU Yinling，WANG Lingmin，CHEN Guangping，MA Jixian，TANG Zhengfu，DAN Zhong，and MU Wanfu*. A New Cauliflower Cultivar‘Yunsonghua 1’ [J]. ACTA HORTICULTURAE SINICA, 2019, 46(8): 1625-1626.
[8]	SONG Xin，TAN Fengquan，ZHANG Miao，CAI Yuankang，GUO Dayong，XIE Kaidong，WU Xiaomeng，and GUO Wenwu*. Metabolic Characteristics of Interspecific Allotetraploid Somatic Hybrid Between‘Newhall’Navel Orange and‘Eureka’Lemon [J]. ACTA HORTICULTURAE SINICA, 2019, 46(1): 37-46.
[9]	SHAN Xiaozheng，WEN Zhenghua，ZHANG Xiaoli，JIANG Hanmin，YAO Xingwei，LIU Lili，NIU Guobao，and SUN Deling*. A New Loose-curd Cauliflower Cultivar‘Yousong 60’ [J]. ACTA HORTICULTURAE SINICA, 2018, 45(S2): 2745-2746.
[10]	YAO Xingwei，SUN Deling，NIU Guobao，SHAN Xiaozheng，WEN Zhenghua，JIANG Hanmin，ZHANG Xiaoli，and LIU Lili*. A New Cauliflower Cultivar‘Jinpin 56’ [J]. ACTA HORTICULTURAE SINICA, 2018, 45(10): 2063-2064.
[11]	DING Yun-hua. A New Cauliflower Cultivar‘Jingsong 1’ [J]. ACTA HORTICULTURAE SINICA, 2016, 43(S2): 2735-2736.
[12]	ZHANG Jie1,2，WANG Gui-xiang2，HAN Shuo2，YAN Hong3，ZONG Mei2，GUO Ning2，and LIU Fan2,*. The Phenotype Evolution and Transfer Breeding of Black Rot Resistance in Somatic Hybrids Between Cauliflower and Black Mustard [J]. ACTA HORTICULTURAE SINICA, 2016, 43(2): 271-280.
[13]	ZHANG Qing-li1，JIN Chuan1，QIN Er-jun1，WANG Yu1，WU Mei1，LI Li-hong1，LI Hui2，CHEN Cheng-bin1，SONG Wen-qin1，and WANG Chun-guo1,*. Cloning and Functional Analysis of a miRNA（Bra-miR07）Associated with the Regulation of Early Organ Development in Cauliflower [J]. ACTA HORTICULTURAE SINICA, 2016, 43(1): 141-150.
[14]	HU Li-Min, TAO Xing-Lin, ZHU Hui-Xia, LIU Ming-Xia. A New Cauliflower Cultivar‘Shengxue 4’ [J]. ACTA HORTICULTURAE SINICA, 2015, 42(5): 1007-1008.
[15]	Lv Jing, LIU Fan, ZONG Mei, HAN Shuo, WANG You-Ping, WANG Gui-Xiang. Identification and Characterization of Introgression and Additional Lines Between Cauliflower and Black Mustard [J]. ACTA HORTICULTURAE SINICA, 2014, 41(3): 456-468.

New Alien Additon Lines Resistance to Black Rot Generated by Somatic Hybridization Between Cauliflower and Black Mustard

PDF

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments